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Microsatellite markers in aquaculture: application in fish population genetics

Microsatellites or Simple Sequence Repeats (SSRs) represent an abundant source of genetic markers which are highly abundant and dispersed evenly throughout eukaryotic genomes. They have become the markers of choice for a wide range of applications in population genetic, conservation and evolutionary...

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Published: 2013
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LEADER 00000njm a2000000a 4500
001 oai:repository.ui.edu.ng:123456789/1123
042 |a dc 
720 |a Mojekwu, T. O.  |e author 
720 |a Anumudu, C. I.  |e author 
260 |c 2013 
520 |a Microsatellites or Simple Sequence Repeats (SSRs) represent an abundant source of genetic markers which are highly abundant and dispersed evenly throughout eukaryotic genomes. They have become the markers of choice for a wide range of applications in population genetic, conservation and evolutionary biology. Microsatellites represent ideal molecular markers because they have multiple alleles which are highly polymorphic among individuals. Polymorphism is achieved by having variable numbers of tandem repeat motifs resulting in size variation which can then be visualized by PCR with pairs of locus-specific flanking primers, followed by electrophoresis of the amplification product. Microsatellite motifs occurs once every 10kb in fishes. They are inherited in a co-dominant fashion, and are fast and easy to assay. They are co-dominant in nature with high levels of polymorphism and can reproduce very well. Hence, giving better information than the dominant marker .This makes them a choice maker for estimating population structure and genetic diversity. 
024 8 |a 2319-2402 
024 8 |a IOSR Journal of Environmental Science, Toxicology and Food Technology 5(4), pp. 43-48 
024 8 |a ui_mojekwu_microsatellite_2013 
024 8 |a http://ir.library.ui.edu.ng/handle/123456789/1123 
245 0 0 |a Microsatellite markers in aquaculture: application in fish population genetics