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Influence of acid-sensing ion channel blocker on behavioral responses in a zebrafish model of acute visceral pain
Acid-sensing ion channels (ASICs) play significant roles in numerous neurological and pathological conditions, including pain. Although acid-induced nociception has been characterized previously in zebrafish, the contri- bution of ASICs in modulating pain-like behaviors is still unknown. Here, we in...
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| Format: | Article |
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2022
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/12072 | ||
| 042 | |a dc | ||
| 720 | |a Adedara, I. A. |e author | ||
| 720 | |a Costa, F. V. |e author | ||
| 720 | |a Biasuz, E. |e author | ||
| 720 | |a Canzian, J. |e author | ||
| 720 | |a Farombi, E. O. |e author | ||
| 720 | |a Rosemberg, D. B. |e author | ||
| 260 | |c 2022 | ||
| 520 | |a Acid-sensing ion channels (ASICs) play significant roles in numerous neurological and pathological conditions, including pain. Although acid-induced nociception has been characterized previously in zebrafish, the contri- bution of ASICs in modulating pain-like behaviors is still unknown. Here, we investigated the role of amiloride, a nonselective ASICs blocker, in the negative modulation of specific behavioral responses in a zebrafish-based model of acute visceral pain. We verified that intraperitoneal injection (i.p.) of 0.25, 0.5, 1.0, and 2.0 mg/mL amiloride alone or vehicle did not change zebrafish behavior compared to saline-treated fish. Administration of 2.5% acetic acid (i.p.) elicited writhing-like response evidenced by the abnormal body curvature and impaired locomotion and motor activity. Attenuation of acetic acid-induced pain was verified at lower amiloride doses (0.25 and 0.5 mg/mL) whereas 1.0 and 2.0 mg/mL abolished pain-like responses. The protective effect of the highest amiloride dose tested was evident in preventing writhing-like responses and impaired locomotion and vertical activity. Collectively, amiloride antagonized abdominal writhing-like phenotype and aberrant behaviors, supporting the involvement of ASICs in a zebrafish-based model of acute visceral pain | ||
| 024 | 8 | |a 0166-4328 | |
| 024 | 8 | |a ui_art_adedara_influence_2022 | |
| 024 | 8 | |a Behavioural Brain Research 416 (113565) | |
| 024 | 8 | |a https://repository.ui.edu.ng/handle/123456789/12072 | |
| 653 | |a Acid-sensing ion channels | ||
| 653 | |a Amiloride | ||
| 653 | |a Pain | ||
| 653 | |a Behavior | ||
| 653 | |a Zebrafish | ||
| 245 | 0 | 0 | |a Influence of acid-sensing ion channel blocker on behavioral responses in a zebrafish model of acute visceral pain |