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Development of a visible spectrophotometric method for the assay of Methyldopa following oxidative coupling with N-(1-naphthyl) ethylenediamine dihydrochloride
Background: Methyldopa is a centrally acting hypertensive drug that remains one of the choice treatment options in the management of hypertension in pregnancy and during surgical anaesthesia. Although a number of methods of analysis are available, most employ sophisticated techniques and strict expe...
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2015
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/12088 | ||
| 042 | |a dc | ||
| 720 | |a Thomas, O. E. |e author | ||
| 720 | |a Mustapha, A. K. |e author | ||
| 260 | |c 2015 | ||
| 520 | |a Background: Methyldopa is a centrally acting hypertensive drug that remains one of the choice treatment options in the management of hypertension in pregnancy and during surgical anaesthesia. Although a number of methods of analysis are available, most employ sophisticated techniques and strict experimental conditions. Objective: To develop and validate a simple visible spectrophotometric analysis for the quantitative determination of methyldopa in bulk and dosage forms. Method: The method was based on the oxidative coupling reaction between methyldopa and N-(1-naphthyl) ethylenediamine (Bratton-Marshall’s reagent) to generate a blue chromogen. Reactions variables critical to optimal response were established. Various analytical and validation parameters including repeatability, reproducibility and selectivity were also determined. Results: The calibration graph was linear between 30 and 80 μg/ml at 580 nm with a correlation coefficient of 0.9996. The apparent molar absorptivity was 1.52 ×103 L mol−1 cm−1 while the limits of detection and quantification were 9.3 and 31 μg/ml respectively. The method was accurate and precise with recovery in the range of 99.26-101.30 % and intra- and inter-day precision (%RSD) at three different concentrations less than 1.0%. When applied to the analysis of dosage form, there was no statistical difference between the new method and the official method. There was no interference from commonly used excipients. Conclusion: The method is rapid, simple and cost-effective. It can serve as a reliable and affordable assay method for the routine analysis of methyldopa in bulk and dosage forms | ||
| 024 | 8 | |a 0189-8434 | |
| 024 | 8 | |a ui_art_thomas_development_2015 | |
| 024 | 8 | |a Nigerian Journal of Pharmaceutical Research 12(1), pp. 147-153. | |
| 024 | 8 | |a https://repository.ui.edu.ng/handle/123456789/12088 | |
| 653 | |a Methyldopa | ||
| 653 | |a Oxidative coupling | ||
| 653 | |a N-(1-naphthyl) ethylenediamine dihydrochloride | ||
| 245 | 0 | 0 | |a Development of a visible spectrophotometric method for the assay of Methyldopa following oxidative coupling with N-(1-naphthyl) ethylenediamine dihydrochloride |