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Normalization of Gene expression by quantitative RT-PCR in human cell line: comparison of 12 endogenous reference genes
BACKGROUND: Polymerase Chain Reaction (PCR) has become an important diagnostic and research tool of modern molecular biology globally. Real-time PCR allows for rapid and reliable quantification of mRNA transcription. Reference genes are used as internal reaction control to normalise mRNA levels betw...
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2018
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