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Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria
The need to investigate the contribution of non-polio enteroviruses to acute flaccid paralysis (AFP) cannot be over emphasized as we move towards a poliovirus free world. Hence, we aim to identify non-polio enteroviruses recovered from the faeces of children diagnosed with AFP in Nigeria.Ninety-six...
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2017
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/12768 | ||
| 042 | |a dc | ||
| 720 | |a Faleye, T. O. C. |e author | ||
| 720 | |a Adewumi, M. O. |e author | ||
| 720 | |a Japhet, M. O. |e author | ||
| 720 | |a David, O. M. |e author | ||
| 720 | |a Oluyege, A. O. |e author | ||
| 720 | |a Adeniji, J. A. |e author | ||
| 720 | |a Famurewa, O. |e author | ||
| 260 | |c 2017 | ||
| 520 | |a The need to investigate the contribution of non-polio enteroviruses to acute flaccid paralysis (AFP) cannot be over emphasized as we move towards a poliovirus free world. Hence, we aim to identify non-polio enteroviruses recovered from the faeces of children diagnosed with AFP in Nigeria.Ninety-six isolates, (95 unidentified and one previously confirmed Sabin poliovirus 3) recovered on RDcell culture from the stool of children <15 years old diagnosed with AFP in 2014 were analyzed. All isolates were subjected to RNA extraction, cDNA synthesis and three different PCR reactions (one panenterovirus 5′-UTR and two different VP1 amplification assays). VP1 amplicons were then sequenced and isolates identified. Results: 92.71% (89/96) of the isolates were detected by at least one of the three assays as an enterovirus. Precisely, 79.17% (76/96), 6.25% (6/96), 7.30% (7/96) and 7.30% (7/96) of the isolates were positive for both, positive and negative, negative and positive, as well as negative for both the 5′-UTR and VP1 assays, respectively. In this study, sixty-nine (69) of the 83 VP1 amplicons sequenced were identified as 27 different enterovirus types. The most commonly detected were CV-B3 (10 isolates) and EV-B75 (5 isolates). Specifically, one, twenty-four and two of the enterovirus types identified in this study belong to EV-A, EV-B and EV-C respectively. This study reports the circulating strains of 27 non-polio enterovirus types in Nigerian children with AFP in 2014 and Nigerian strains of CV-B2, CV-B4, E17, EV-B80, EV-B73, EV-B97, EV-B93, EV-C99 and EV-A120 were reported for the first time. Furthermore, it shows that being positive for the 5′-UTR assay should not be the basis for subjecting isolates to the VP1 assays. | ||
| 024 | 8 | |a 1743-422X | |
| 024 | 8 | |a ui_art_falaye_non-polio_2017 | |
| 024 | 8 | |a Virology Journal 14(175), pp. 14-34 | |
| 024 | 8 | |a https://repository.ui.edu.ng/handle/123456789/12768 | |
| 653 | |a AFP | ||
| 653 | |a Enteroviruses | ||
| 653 | |a Nigeria | ||
| 653 | |a Non-polio enteroviruses | ||
| 653 | |a VP1 analysis | ||
| 245 | 0 | 0 | |a Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria |