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A possible risk of environmental exposure to HEV in Ibadan, Oyo State, Nigeria
"Hepatitis E virus (HEV) infection is both a major public health concern and emerging global health concern, with a documented incidence of 20 million, 3.4 million clinical cases, 70,000 deaths, and 3,000 stillbirths. The aetiologic agent, HEV is a primarily enterally transmitted hepatotropic virus....
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| Format: | Article |
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2020-08-13
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/12824 | ||
| 042 | |a dc | ||
| 720 | |a Olayinka, A. |e author | ||
| 720 | |a Ifeorah, I. M. |e author | ||
| 720 | |a Omotosho, O. |e author | ||
| 720 | |a Faleye, T. O. C. |e author | ||
| 720 | |a Odukaye, O. |e author | ||
| 720 | |a Bolaji, O. |e author | ||
| 720 | |a Ibitoye, I. |e author | ||
| 720 | |a Ope-Ewe, O. |e author | ||
| 720 | |a Adewumi, M. O. |e author | ||
| 720 | |a Adeniji, J. A. |e author | ||
| 260 | |c 2020-08-13 | ||
| 520 | |a "Hepatitis E virus (HEV) infection is both a major public health concern and emerging global health concern, with a documented incidence of 20 million, 3.4 million clinical cases, 70,000 deaths, and 3,000 stillbirths. The aetiologic agent, HEV is a primarily enterally transmitted hepatotropic virus. Fecal samples were collected from three selected pig farms across Ibadan, South-west Nigeria. Randomly picked samples were pooled per unit pen and fecal suspensions prepared were subjected to HEV Antigen (Ag) enzyme-linked immunosorbent assay. Molecular probing was done by Reverse Transcription and nested polymerase reaction (RT-nPCR) and deep sequencing. Sequencing was done paired-end for 300 cycles using the HiSeq system. Overall farm prevalence of 66.7% (2/3) and prevalence at individual level of 13.2% (9/68) were recorded. All nine samples positive for the ELISA screen were negative when subjected to RT-nPCR assays. Further, on deep sequencing, no HEV genomic fragment was found in the sample using de-novo assembly. Findings suggest possibly inapparent HEV in the pigs studied or a yet to be identified protein with HEV-Ag cross-reactivity ability on ELISA, thus constituting a possible risk of exposure to HEV infection in the population. Consequently, we recommend prompt intervention to unravel the mystery and break the chain of transmission. | ||
| 024 | 8 | |a 1532-1819 | |
| 024 | 8 | |a 1532-1819 | |
| 024 | 8 | |a Journal of Immunoassay and Immunochemistry 41(5), pp.875–884 | |
| 024 | 8 | |a ui_art_olayinka_possible_2020 | |
| 024 | 8 | |a https://repository.ui.edu.ng/handle/123456789/12824 | |
| 653 | |a Pig | ||
| 653 | |a HEV antigen | ||
| 653 | |a hepatitis E virus | ||
| 653 | |a HEV RNA | ||
| 653 | |a Nigeria | ||
| 653 | |a viral hepatitis | ||
| 245 | 0 | 0 | |a A possible risk of environmental exposure to HEV in Ibadan, Oyo State, Nigeria |