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An improved PCR method for detection of HIV-1 proviral DNA of a wide range of subtypes and recombinant forms circulating globally
Proviral DNAs are being measured increasingly as a marker of the efficacy of highly active anti-retroviral therapy (HAART) and is accepted for the early diagnosis of perinatal HIV-1 infections. This requires a standardized test which enables the detection of a wide range of subtypes worldwide includ...
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2011
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/2496 | ||
| 042 | |a dc | ||
| 720 | |a Weinder, J. |e author | ||
| 720 | |a Cassens, U. |e author | ||
| 720 | |a Gohde, W. |e author | ||
| 720 | |a Sibrowski, W. |e author | ||
| 720 | |a Odaibo, G. |e author | ||
| 720 | |a Olaleye, D. |e author | ||
| 720 | |a Reichelt, D. |e author | ||
| 720 | |a Greve, B. |e author | ||
| 260 | |c 2011 | ||
| 520 | |a Proviral DNAs are being measured increasingly as a marker of the efficacy of highly active anti-retroviral therapy (HAART) and is accepted for the early diagnosis of perinatal HIV-1 infections. This requires a standardized test which enables the detection of a wide range of subtypes worldwide including O, N and circulating recombinant forms (CRFs). Based on a previous publication, a PCR - Test for HIV-1 provirus detection in peripheral blood mononuclear cells (PBMCs) was developed. Blood samples from 80 individuals infected with HIV-1 and 20 persons negative for HIV-1&2 from Africa and Germany were tested for the presence of HIV-1 provirus DNA. The primer system used enables the detection of proviral DNA despite the high concentrations of human DNA. The limit of detection was determined to be 5 copies per 10(5) cells. All 20 samples from persons negative for HIV were negative for HIV-1 proviral DNA while provirus DNA was amplified from 76 of the 80 (95%) samples from persons infected with HIV. The amplified products were detected by gel-electrophoresis, flow cytometry and real-time PCR. All three detection systems provided the same results. | ||
| 024 | 8 | |a Journal of Virological Methods 172,pp. 22-26 | |
| 024 | 8 | |a http://ir.library.ui.edu.ng/handle/123456789/2496 | |
| 245 | 0 | 0 | |a An improved PCR method for detection of HIV-1 proviral DNA of a wide range of subtypes and recombinant forms circulating globally |