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Phytosterols from spondias mombin Linn with antimycobacterial activities
The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of Spondias mombin was evaluated for its in vitro activity against Mycobacterium tuberculosis (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by...
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2013-01
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/2995 | ||
| 042 | |a dc | ||
| 720 | |a Olugbuyiro, J. A. O. |e author | ||
| 720 | |a Moody, J. O. |e author | ||
| 720 | |a Hamann, M. T. |e author | ||
| 260 | |c 2013-01 | ||
| 520 | |a The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of Spondias mombin was evaluated for its in vitro activity against Mycobacterium tuberculosis (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (230-400 mesh) and purification was done using HPLC and TLC. In vitro antimycobacterial susceptibility was performed by a fluorometric microplate alamar blue assay (MABA) and percentage mycobacterial inhibition was calculated. The structures of the isolated compounds were established by spectroscopic analysis. The active VLC fraction exhibited 91% inhibition against M. tuberculosis H37Rv at a concentration of 40 μg/mL. The HPLC fraction SMi-15 containing compounds 1 and 2 showed 92.8% inhibition against M. tuberculosis. Two new antimycobacterial phytosterols were isolated from the stem bark of S. mombin and the structureswere identified as mombintane I (1) and mombintane II (2). The stem bark extractives of S. mombin contain antitubercular principles of the class phytosterol and support an important potential of triterpenoids. | ||
| 024 | 8 | |a 1119-5096 | |
| 024 | 8 | |a African Journal Biomedical Research 16(1), pp. 19- 24 | |
| 024 | 8 | |a ui_art_olugbuyiro_Phytosterols_2013 | |
| 024 | 8 | |a http://ir.library.ui.edu.ng/handle/123456789/2995 | |
| 653 | |a Spondias mombin | ||
| 653 | |a mycobacterium tuberculosis | ||
| 653 | |a antimycobacterial | ||
| 653 | |a mombintane I | ||
| 653 | |a mombintane II | ||
| 245 | 0 | 0 | |a Phytosterols from spondias mombin Linn with antimycobacterial activities |