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Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection
Dissertation (MSc (Genetics))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613552338010112 |
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| access_status_str | Open Access |
| author2 | Van den Berg, Noelani |
| author_browse | Van den Berg, Noelani |
| author_facet | Van den Berg, Noelani |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/100387 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:57.427Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/100387 Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection Van den Berg, Noelani susanna.anbu@up.ac.za Swart, Velushka Anbu, Susanna Pearl UCTD Sustainable Development Goals (SDGs) Phytophthora root rot (PRR) Avocado NLR proteins Dusa® rootstock resistance Effector-triggered immunity (ETI) Hypersensitive response (HR) Nicotiana benthamiana model Dissertation (MSc (Genetics))--University of Pretoria, 2024. Avocado (Persea americana) production is threatened by Phytophthora cinnamomi, the causative agent of Phytophthora root rot (PRR). Current control measures rely on phosphite-based fungicides and partially-resistant rootstocks like Dusa®. However, the immune mechanisms underlying Dusa®'s resistance remain unclear. This study investigated nucleotide-binding leucine-rich repeat (NLR) proteins in Dusa®, which play a critical role in effector-triggered immunity (ETI) and hypersensitive response (HR). Using a Nicotiana benthamiana-P. cinnamomi model system, we identified the biotrophic and necrotrophic phases of infection, applying this knowledge to study avocado NLRs. Six PaNLR candidates were selected based on expression patterns, defense motifs, and effector recognition potential. Transient expression assays revealed that five PaNLRs caused HR only upon pathogen introduction, while one induced cell death independently. Cellular localization studies showed most PaNLRs reside in the nucleus during the resting stage, with one in the cytoplasm. Upon P. cinnamomi infection, all candidates triggered a strong HR, effectively restricting pathogen spread. These findings suggest that PaNLRs adopt diverse strategies to induce immunogenic cell death, balancing resource conservation and rapid defense activation. This study provides the first molecular characterization of PaNLRs, offering insights into avocado immunity and pathways for enhancing resistance in this economically important crop. Hans Merensky Legacy Foundation Biochemistry, Genetics and Microbiology (BGM) Msc (Genetics) Unrestricted Faculty of Natural and Agricultural Sciences SDG-02: Zero hunger SDG-12: Responsible consumption and production SDG-15: Life on land 2025-01-29T13:46:15Z 2025-01-29T13:46:15Z 2025-04 2024-11 Dissertation * A2025 http://hdl.handle.net/2263/100387 https://10.25403/UPresearchdata.28296932 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Phytophthora root rot (PRR) Avocado NLR proteins Dusa® rootstock resistance Effector-triggered immunity (ETI) Hypersensitive response (HR) Nicotiana benthamiana model Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection |
| title | Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection |
| title_full | Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection |
| title_fullStr | Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection |
| title_full_unstemmed | Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection |
| title_short | Functional characterization of candidate Persea americana nucleotide-binding leucine rich repeat (PaNLR) genes during Phytophthora cinnamomi infection |
| title_sort | functional characterization of candidate persea americana nucleotide binding leucine rich repeat panlr genes during phytophthora cinnamomi infection |
| topic | UCTD Sustainable Development Goals (SDGs) Phytophthora root rot (PRR) Avocado NLR proteins Dusa® rootstock resistance Effector-triggered immunity (ETI) Hypersensitive response (HR) Nicotiana benthamiana model |
| url | http://hdl.handle.net/2263/100387 https://10.25403/UPresearchdata.28296932 |