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Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus
Thesis (PhD (Genetics))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613515824496640 |
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| access_status_str | Open Access |
| author2 | Barnes, Irene |
| author_browse | Barnes, Irene |
| author_facet | Barnes, Irene |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Genetics))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/100471 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:22.997Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/100471 Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus Barnes, Irene u14049423@tuks.co.za Wingfield, Michael J. Lynn, Kira Mary Theresa UCTD Sustainable Development Goals (SDGs) Ceratocystis Forestry Plant pathology Entomology Diagnostics Thesis (PhD (Genetics))--University of Pretoria, 2024. In this thesis, Detection, dispersal, and global distribution of Ceratocystis species on Eucalyptus, the candidate investigated the species boundaries and transmission mechanisms of two economically important fungal pathogens. Utilizing a dataset of 1174 isolates collected from 11 countries and 8 plant hosts, population genetics, phylogenetic analysis, and morphological comparisons were employed to confirm that Ceratocystis manginecans and C. eucalypticola are distinct species, resolving long-standing taxonomic confusion. Hybridization events between these species were also identified clarifying ambiguity in the literature. The candidate developed a cutting-edge qPCR-based HRMA diagnostic tool enabling rapid species identification of these fungal pathogens directly from infected plant material and fungal cultures. This tool was successfully applied to reveal key dispersal mechanisms, including insect-mediated and airborne transmission. These findings offer valuable insights into pathogen detection, dispersal, and species boundaries, providing crucial tools for effectively managing the spread of these economically significant pathogens within the global forestry industry. RGE-FABI Tree Health Programme and the National Research Foundation : (NRF, MND190619448979) Biochemistry, Genetics and Microbiology (BGM) PhD (Genetics) Unrestricted Faculty of Natural and Agricultural Sciences None 2025-02-03T23:02:08Z 2025-02-03T23:02:08Z 2025-05 2024-10 Thesis * A2025 http://hdl.handle.net/2263/100471 https://doi.org/10.25403/UPresearchdata.28284677 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Ceratocystis Forestry Plant pathology Entomology Diagnostics Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus |
| title | Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus |
| title_full | Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus |
| title_fullStr | Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus |
| title_full_unstemmed | Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus |
| title_short | Detection, dispersal and global distribution of Ceratocystis species on Eucalyptus |
| title_sort | detection dispersal and global distribution of ceratocystis species on eucalyptus |
| topic | UCTD Sustainable Development Goals (SDGs) Ceratocystis Forestry Plant pathology Entomology Diagnostics |
| url | http://hdl.handle.net/2263/100471 https://doi.org/10.25403/UPresearchdata.28284677 |