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Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa
Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2024.
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University of Pretoria
2025
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| _version_ | 1867613675943100416 |
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| access_status_str | Open Access |
| author2 | Ehlers, M.M. (Marthie Magdaleen) |
| author_browse | Ehlers, M.M. (Marthie Magdaleen) |
| author_facet | Ehlers, M.M. (Marthie Magdaleen) |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/100684 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:55.528Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/100684 Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa Ehlers, M.M. (Marthie Magdaleen) tatianangoie17@gmail.com Hamiwe, Thabo Kingsburgh, Chanel Ngoie, Kazadi UCTD Staphylococcus aureus Plasmid Horizontal gene transfer Antibiotic resistance Methicillin-susceptible Staphylococcus aureus Methicillin-resistant Staphylococcus aureus Rep genes Virulence genes Whole genome sequencing (WGS) PCR-based replicon typing Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2024. OBJECTIVES : Replication initiation (rep) genes found in plasmids have been linked to antibiotic resistance in bacteria. This study aimed to explore the relationship between plasmid diversity, antibiotic resistance profiles and the presence of virulence genes in S. aureus isolates from private hospitals in Gauteng, South Africa. METHODS : Automated antibiotic susceptibility testing was done on 100 taphylococcus aureus isolates. Plasmid DNA extraction and polymerase chain reaction (PCR)-based replicon typing were done on all isolates. Multiplex-PCR was used to screen for virulence genes in plasmid positive isolates. Whole genome sequencing (WGS) was conducted on 10 representative isolates to assess genetic diversity. RESULTS : The most common phenotypic antibiotic resistances observed were erythromycin 29% (29/100), clindamycin 26% (26/100) and ciprofloxacin 15% (15/100). Plasmids were found in 60% (60/100) of the isolates. Associations were observed between: rep7a (pS0385p1) with tetracycline (tetK) and rep7a (pTZ4) with chloramphenicol (cat) resistance, rep10a (pDLK1) with macrolide, lincosamide and streptogramin (ermC) resistance and rep5 (pN315) and rep16 (pSaaS6159) with penicillin (blaZ) resistance. No specific virulence gene could be associated with a particular rep gene. Sequence types (ST) associated with epidemics (ST22 and ST36), foodborne outbreaks (ST6) and zoonosis (ST1 and ST152) were identified by WGS. The ST1, ST6, ST22 and ST36 were identified in one isolate each, while ST152 was identified in two isolates. CONCLUSIONS : These findings highlighted an association between certain plasmids (pS0385p1, pTZ4, pDLK1, pN315, and pSaaS6159) and ARG. Enhanced surveillance and antibiotic stewardship programs in South Africa focusing on these plasmids could help better understand the spread of antibiotic resistance in healthcare settings. National Research Foundation Bursary Medical Microbiology MSc (Medical Microbiology) Unrestricted Faculty of Health Sciences None 2025-02-11T10:28:08Z 2025-02-11T10:28:08Z 2025-04 2024-11 Dissertation * A2025 http://hdl.handle.net/2263/100684 https://doi.org/10.25403/UPresearchdata.28378013.v1 https://doi.org/10.25403/UPresearchdata.28378013 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Staphylococcus aureus Plasmid Horizontal gene transfer Antibiotic resistance Methicillin-susceptible Staphylococcus aureus Methicillin-resistant Staphylococcus aureus Rep genes Virulence genes Whole genome sequencing (WGS) PCR-based replicon typing Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa |
| title | Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa |
| title_full | Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa |
| title_fullStr | Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa |
| title_full_unstemmed | Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa |
| title_short | Relationship between the molecular diversity of plasmids, antibiotic resistance and virulence genes of Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa |
| title_sort | relationship between the molecular diversity of plasmids antibiotic resistance and virulence genes of staphylococcus aureus isolates from private hospitals in gauteng south africa |
| topic | UCTD Staphylococcus aureus Plasmid Horizontal gene transfer Antibiotic resistance Methicillin-susceptible Staphylococcus aureus Methicillin-resistant Staphylococcus aureus Rep genes Virulence genes Whole genome sequencing (WGS) PCR-based replicon typing |
| url | http://hdl.handle.net/2263/100684 https://doi.org/10.25403/UPresearchdata.28378013.v1 https://doi.org/10.25403/UPresearchdata.28378013 |