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The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats
Dissertation (MSc (Human Physiology))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613438980653056 |
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| access_status_str | Open Access |
| author2 | Kasonga, Abe E. |
| author_browse | Kasonga, Abe E. |
| author_facet | Kasonga, Abe E. |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Human Physiology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/100697 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:09.504Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/100697 The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats Kasonga, Abe E. u15032312@tuks.co.za Nyakudya, Trevor Tapiwa Mason, Caitlin UCTD Sustainable Development Goals (SDGs) Chrysin Osteoclastogenesis Micro-CT Receptor activator of nuclear factor kappa-B ligand Western blot Dissertation (MSc (Human Physiology))--University of Pretoria, 2024. Bone is regarded as a metabolically active tissue that is constantly being reformed and resorbed by osteoblasts and osteoclasts. Abnormal increases in osteoclast productivity can result in bone deterioration. Receptor activator of nuclear factor-kappa B (RANK) signalling is critical for function and formation of osteoclasts. This study aimed to investigate the potential beneficial effects on bone from chrysin, a bioactive phytochemical. Western blotting was carried out to assess the impact of chrysin on the RANK signalling pathways. Immunofluorescence was conducted to determine how chrysin effects the translocation of nuclear factor kappa B (NFκB) to the nucleus. Micro-computed tomography (microCT) was utilised to assess the tibia bones in Sprague-Dawley rats. The parameters evaluated of the whole bone, length, volume, surface area, bone volume fraction, segmented surface density, and the thickness, number, and spacing of trabecular bone. The parameters evaluated for the midpoint of the bone, volume, area, bone volume fraction, segmented surface density, thickest and thinnest part of the bone midpoint. The IC50 (14µM) was determined in a pilot study performed previously. Western blotting revealed no significant changes in the phosphorylation of mitogen-activated protein kinases (MAPKs), specifically ERK, JNK, and P38, and NFκB signally pathways when treated to chrysin. Immunofluorescence investigation showed a significant inhibition in the amount of NFκB that translocated to the nucleus from the cytoplasm when treated to chrysin, when compared to the positive RANK ligand (RANKL) control. Micro-CT analysis of tibial bones indicated no significant differences in most of the parameters analysed except when an unpaired T-test was performed comparing the control group and the chrysin treated group. Chrysin significantly increased the area, volume, and segmented bone density of the midpoint of the bones. Overall, the findings suggest that chrysin inhibits osteoclastogenesis via the NFκB signalling pathway, by inhibiting the translocation from the cytoplasm to the nucleus. These findings highlight chrysin’s potential as a viable therapeutic agent in bone degenerative disorders. National Research Foundation of South Africa, Thuthuka Fund (Grant holder to Dr A.E. Kasonga: 121828) Physiology MSc (Human Physiology) Unrestricted Faculty of Health Sciences SDG-03: Good health and well-being 2025-02-11T12:16:49Z 2025-02-11T12:16:49Z 2025-05-01 2024-07-31 Dissertation * A2025 http://hdl.handle.net/2263/100697 https://doi.org/10.25403/UPresearchdata.28380680 https://doi.org/10.25403/UPresearchdata.28380680 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Chrysin Osteoclastogenesis Micro-CT Receptor activator of nuclear factor kappa-B ligand Western blot The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats |
| title | The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats |
| title_full | The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats |
| title_fullStr | The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats |
| title_full_unstemmed | The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats |
| title_short | The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats |
| title_sort | effects of chrysin on osteoclastogenesis in raw264 7 murine macrophages and bone metabolism in sprague dawley rats |
| topic | UCTD Sustainable Development Goals (SDGs) Chrysin Osteoclastogenesis Micro-CT Receptor activator of nuclear factor kappa-B ligand Western blot |
| url | http://hdl.handle.net/2263/100697 https://doi.org/10.25403/UPresearchdata.28380680 https://doi.org/10.25403/UPresearchdata.28380680 |