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Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner

Dissertation (MSc)--University of Pretoria, 2024.

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Other Authors: L'Abbé, Ericka Noëlle
Format: Thesis
Language:English
Published: University of Pretoria 2025
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access_status_str Open Access
author2 L'Abbé, Ericka Noëlle
author_browse L'Abbé, Ericka Noëlle
author_facet L'Abbé, Ericka Noëlle
collection Thesis
dc_rights_str_mv © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc)--University of Pretoria, 2024.
format Thesis
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institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:38:38.583Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/100878 Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner L'Abbé, Ericka Noëlle pieter.dewet@tuks.co.za Ridel, Alison Fany De Wet, Pieter Daniël UCTD Sustainable Development Goals (SDGs) Saw mark analysis Forensic anthropology Stereomicroscope analysis Micro-XCT 3D printing Dissertation (MSc)--University of Pretoria, 2024. Saw mark analysis is a vital component of forensic anthropology, particularly in the investigation of post-mortem dismemberments. Despite its medicolegal significance, this field remains underdeveloped in South Africa, where a high incidence of murder, including dismemberments, occurs annually. The scarcity of trained practitioners capable of analysing saw marks often results in the loss of critical evidence regarding the tools used in these crimes. This study aimed to evaluate and compare saw mark features created by a 6 tooth per inch (TPI) crosscut saw on human femora using a stereomicroscope and micro-focus X-ray computed tomography (micro-XCT) modality. It also sought to validate the use of three-dimensional (3D) printed saw mark replicas for courtroom presentations and educational purposes. By addressing these objectives, the research advances saw mark analysis methodologies and their practical applications in forensic contexts. Four fresh human femora were obtained from the National Tissue Bank of South Africa, defleshed, and sectioned. Thirty-six bone sections were prepared, yielding 32 false starts and 64 complete cuts. Analyses were conducted using a stereomicroscope, micro-XCT, and 3D print modality, assessing 16 unique saw mark features. Statistical evaluations included reproducibility assessments, modality comparisons, and validation of 3D printed models. Intra- and inter-observer error rates were calculated using Cohen’s Kappa, proportion of agreement, and technical error of measurement. Comparative analyses were performed using Chi-Squared tests, Paired Student’s t-tests, and Wilcoxon signed-rank tests. Reproducibility assessments demonstrated high intra- and inter-observer agreement for most false start and complete cut features, although agreement varied by modality. Some features, such as minimum kerf width (MKW), kerf wall shape, and kerf floor shape, showed perfect or near-perfect agreement across all modalities. In contrast, features such as pull-out striae, kerf flare, and entrance shaving exhibited lower agreement, particularly in 3D print evaluations. Measurement reproducibility was highest for the micro-XCT modality, followed by the stereomicroscope and 3D prints modalities, with intra-observer measurements yielding lower error rates than inter-observer measurements. Modality analysis revealed that stereomicroscope and micro-XCT analysis offered complementary strengths, providing detailed visualisation of nuanced features, whereas 3D prints were less reliable for capturing fine details. Statistical validation highlighted the limited comparability between 3D prints and stereomicroscopic analysis of complex features. Stereomicroscope analysis emerged as the most effective modality for detecting detailed saw mark features, followed by micro-XCT, whereas 3D prints demonstrated limitations in replicating subtle textures. Minimum kerf width was consistently measurable across all modalities, but complex features, such as exit chipping and exit shaving, showed better agreement between the stereomicroscope and micro-XCT modalities than with 3D prints. Significant differences across modalities underscore the importance of using analytical methods in obtaining reliable results. While 3D prints have the potential for teaching and demonstrations, they require further refinement to accurately replicate intricate saw mark features. Future research should focus on standardising the measurement methodologies for complex features to improve reproducibility and consistency. Higher resolution micro-XCT scanning may enhance the non-destructive visualisation of fine details. Additionally, studies on a broader range of saw types could deepen the understanding of feature variations across tool classes and modalities, thereby strengthening forensic applications. Department of Sport, Arts & Culture (DSAC) Anatomy MSc (Anatomy) Unrestricted Faculty of Health Sciences SDG-09: Industry, innovation and infrastructure SDG-16: Peace, justice and strong institutions 2025-02-13T15:35:28Z 2025-02-13T15:35:28Z 2025 2024 Dissertation * A2025 http://hdl.handle.net/2263/100878 10.25403/UPresearchdata.28408436 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Sustainable Development Goals (SDGs)
Saw mark analysis
Forensic anthropology
Stereomicroscope analysis
Micro-XCT
3D printing
Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner
title Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner
title_full Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner
title_fullStr Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner
title_full_unstemmed Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner
title_short Evaluation and comparison of saw mark features using a stereomicroscope and micro-XCT scanner
title_sort evaluation and comparison of saw mark features using a stereomicroscope and micro xct scanner
topic UCTD
Sustainable Development Goals (SDGs)
Saw mark analysis
Forensic anthropology
Stereomicroscope analysis
Micro-XCT
3D printing
url http://hdl.handle.net/2263/100878