Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome
Dissertation (MSc (Medical Immunology))-- University of Pretoria, 2024.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2025
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613707712856064 |
|---|---|
| access_status_str | Open Access |
| author2 | Meyer, Pieter Willem Adriaan |
| author_browse | Meyer, Pieter Willem Adriaan |
| author_facet | Meyer, Pieter Willem Adriaan |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Immunology))-- University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/101029 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:25.890Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/101029 The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome Meyer, Pieter Willem Adriaan dvruler@gmail.com Rossouw, Theresa M. Van Ruler, Divan UCTD Sustainable Development Goals (SDGs) Immune reconstitution inflammatory syndrome (IRIS) Human immunodeficiency virus (HIV) Inflammation Micro-RNA (ribonucleic acid) CD4 Viral load Biomarkers Machine learning K-Nearest Neighbours Diagnosis Dissertation (MSc (Medical Immunology))-- University of Pretoria, 2024. Immune Reconstitution Inflammatory Syndrome (IRIS) arises from the restoration of antigen-specific immune responses following antiretroviral therapy (ART) and presents in two forms: paradoxical and unmasking IRIS. Currently, no definitive diagnostic method exists for IRIS, and diagnosis relies on clinical symptoms in the context of immunological and virological responses to ART. This study hypothesized that specific microRNAs (miRNAs) involved in viral control and inflammation could be informative in identifying IRIS. We investigated the expression of 10 candidate miRNAs (miR-155, miR-132a, miR-10a, miR-146a, miR-146b, miR-181a, miR-27a, and miR-375, miR-16-5p, miR-191-5p) in 28 HIV-positive patients before ART, at approximately six months post-ART, and upon development of inflammatory symptoms. Significant differential expression of miR-146a and miR-181a was observed in IRIS patients compared to those without IRIS. Using pre-ART CD4 count and viral load data, in addition to miR-146a and miR-181a expression data, 7 patients who presented with ambiguous cases could be classified into IRIS and non-IRIS groups. Of these 7 patients, 3 were classified as having developed IRIS. These findings suggest that miR-146a and miR-181a may serve as potential biomarkers for diagnosing or predicting IRIS, though further research is required to confirm their clinical utility. RESCOM Immunology MSc (Medical Immunology) Unrestricted Faculty of Health Sciences SDG-03: Good health and well-being 2025-02-18T14:32:21Z 2025-02-18T14:32:21Z 2025-05-31 2024-12-06 Dissertation * May 2024 http://hdl.handle.net/2263/101029 https://doi.org/10.25403/UPresearchdata.28435820 10.25403/UPresearchdata.28435820 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Immune reconstitution inflammatory syndrome (IRIS) Human immunodeficiency virus (HIV) Inflammation Micro-RNA (ribonucleic acid) CD4 Viral load Biomarkers Machine learning K-Nearest Neighbours Diagnosis The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome |
| title | The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome |
| title_full | The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome |
| title_fullStr | The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome |
| title_full_unstemmed | The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome |
| title_short | The role of micro-RNA in the diagnosis of HIV-associated immune reconstitution inflammatory syndrome |
| title_sort | role of micro rna in the diagnosis of hiv associated immune reconstitution inflammatory syndrome |
| topic | UCTD Sustainable Development Goals (SDGs) Immune reconstitution inflammatory syndrome (IRIS) Human immunodeficiency virus (HIV) Inflammation Micro-RNA (ribonucleic acid) CD4 Viral load Biomarkers Machine learning K-Nearest Neighbours Diagnosis |
| url | http://hdl.handle.net/2263/101029 https://doi.org/10.25403/UPresearchdata.28435820 |