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Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model
Dissertation (MSc (Anatomy))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613707653087232 |
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| access_status_str | Open Access |
| author2 | Oberholzer, Hester Magdalena |
| author_browse | Oberholzer, Hester Magdalena |
| author_facet | Oberholzer, Hester Magdalena |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Anatomy))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/101049 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:25.890Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/101049 Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model Oberholzer, Hester Magdalena u18082892@tuks.co.za Van Rooy, Mia-Jeanne Serem, June Cheptoo Masetla, Felicia Maite UCTD Sustainable Development Goals (SDGs) Sprague Dawley Lungs Streptozotocin Inflammation Oxidative stress Dissertation (MSc (Anatomy))--University of Pretoria, 2024. Streptozotocin (STZ) is a commonly used compound for the induction of type 2 diabetes mellitus (T2DM) in animal models, through its cytotoxic effect on pancreatic cells. However, its acute effects on non-pancreatic tissues like the lungs are not well understood. This study aimed to examine the histopathological impact of STZ on the lungs of male Sprague-Dawley rats. The rats were divided into two groups: a control group on a normal diet and an experimental group receiving a high-fat diet and 10% sucrose water for 8 weeks, followed by an STZ injection (30 mg/kg body weight). All the rats were terminated 9 days after STZ administration, and lung samples were collected for analyses using light microscopy, transmission electron microscopy (TEM), immunohistochemistry (IHC) and confocal fluorescence laser scanning microscopy (CFLSM). Light microscopy revealed alterations in lung structure including thickening of alveolar septa, narrowing of alveoli, and inflammatory infiltrates in the STZ group. TEM revealed mitochondrial damage in type 2 pneumocytes, including membrane fragmentation, cristae loss, and formation of mitochondrial-derived vesicles. Immunohistochemistry results indicated the presence of both oxidative stress (OS) using markers 8-Hydroxyguanosine and nitrotyrosine and inflammation using markers TNF-α and IL-6 in the experimental groups. Confocal microscopy revealed a significantly higher expression of myeloperoxidase, neutrophil elastase, and citrullinated histone 3 in the STZ group compared to controls. These findings suggest that STZ induces considerable lung damage, emphasizing the need to consider lung toxicity in studies involving STZ. National Research Foundation (NRF) Anatomy MSc (Anatomy) Unrestricted Faculty of Health Sciences SDG-03: Good health and well-being 2025-02-19T10:28:09Z 2025-02-19T10:28:09Z 2025-04 2024-12 Dissertation * A2025 http://hdl.handle.net/2263/101049 https://doi.org/10.25403/UPresearchdata.28437017 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Sprague Dawley Lungs Streptozotocin Inflammation Oxidative stress Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model |
| title | Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model |
| title_full | Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model |
| title_fullStr | Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model |
| title_full_unstemmed | Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model |
| title_short | Lung-based evaluation of oxidative stress and inflammation in an established STZ Sprague-Dawley rat model |
| title_sort | lung based evaluation of oxidative stress and inflammation in an established stz sprague dawley rat model |
| topic | UCTD Sustainable Development Goals (SDGs) Sprague Dawley Lungs Streptozotocin Inflammation Oxidative stress |
| url | http://hdl.handle.net/2263/101049 https://doi.org/10.25403/UPresearchdata.28437017 |