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Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio
Dissertation (MSc (Genetics))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613522046746624 |
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| access_status_str | Open Access |
| author2 | Slippers, Bernard |
| author_browse | Slippers, Bernard |
| author_facet | Slippers, Bernard |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/101093 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:28.861Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/101093 Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio Slippers, Bernard hannes.strydom1995@gmail.com Chapwanya, Michael Ouifiki, Rachid Strydom, Hannes UCTD Sustainable Development Goals (SDGs) Invasive pests Sirex noctilio Gene drive CRISPR-Cas Mathematical modelling Dissertation (MSc (Genetics))--University of Pretoria, 2024. Sirex noctilio is an invasive pest of pine that has caused significant economic damage in South Africa and many other Southern Hemisphere countries. Current management tools are not efficient in all cases and consequently there is a need for more efficient and targeted control measures. An emerging tool for pest management is the use of gene editing and associated gene drive systems. In this study, we aim to investigate the use of CRISPR-Cas gene drive systems in the management of S. noctilio in South Africa. As a first step, we developed a model for the population dynamics of S. noctilio, using historical national population monitoring data and incorporating the influence of two main biological control agents of the pest. We then modelled the influence of two different CRISPR-Cas systems on the population dynamics of S. noctilio namely, a baseline CRISPR model and Complementary Sex Determination CRISPR (CSD) model. Each model is used to simulate a male and female only introduction strategy to estimate the effectiveness of different methods of introducing the gene drive system. Results suggest that both CRISPR gene drive system would be effective at controlling the population growth of S. noctilio at high levels of introduction, but overall population control would be hindered by practical limitations. Although only two CRISPR models were explored, the underlying population model serves as a framework for further studies into the population dynamics of Sirex noctilio, as well as many other CRISPR-Cas gene drive systems. Biochemistry, Genetics and Microbiology (BGM) MSc (Genetics) Unrestricted Faculty of Natural and Agricultural Sciences None 2025-02-20T14:29:07Z 2025-02-20T14:29:07Z 2025-04 2024-11 Dissertation * A2025 http://hdl.handle.net/2263/101093 10.25403/UPresearchdata.28450352 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Invasive pests Sirex noctilio Gene drive CRISPR-Cas Mathematical modelling Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio |
| title | Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio |
| title_full | Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio |
| title_fullStr | Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio |
| title_full_unstemmed | Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio |
| title_short | Modelling the population dynamics of CRISPR-Cas9 gene drive systems in Sirex noctilio |
| title_sort | modelling the population dynamics of crispr cas9 gene drive systems in sirex noctilio |
| topic | UCTD Sustainable Development Goals (SDGs) Invasive pests Sirex noctilio Gene drive CRISPR-Cas Mathematical modelling |
| url | http://hdl.handle.net/2263/101093 |