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Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers
Mini Dissertation (MSc (Global One Health))--University of Pretoria, 2025.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613541452742656 |
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| access_status_str | Open Access |
| author2 | Keddy, Karen H. |
| author_browse | Keddy, Karen H. |
| author_facet | Keddy, Karen H. |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Mini Dissertation (MSc (Global One Health))--University of Pretoria, 2025. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/101861 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:45.339Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/101861 Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers Keddy, Karen H. u22899317@tuks.co.za Luwe, Michael. (Prince Aaron) UCTD Sustainable Development Goals (SDGs) Escherichia coli Enterococcus Food safety Knowledge, attitudes and practices (KAP) Northern Malawi Mini Dissertation (MSc (Global One Health))--University of Pretoria, 2025. The prevalence of multidrug-resistant (MDR) opportunistic pathogens in many of the sub-Saharan countries has been one of the compelling reasons for proper mapping and management of the disease in hotspot areas. This study investigated the prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species isolated from pigs in the northern part of Malawi and analysed the knowledge, attitudes and practices (KAP) of butchers concerning good hygienic practices. The KAP questionnaire was administered to 75 participants and 232 caecal samples were collected from pigs slaughtered at randomly selected slaughter places in Nkhata Bay, Rumphi, Mzimba, Karonga, and Mzuzu city. Overall KAP scores for knowledge, attitude, and practices were 81%, 73% and 46%, respectively. A positive correlation between the butchers’ knowledge and their attitude (r = .46, p<0.001), knowledge and practices (r = .38, p<0.001), and attitude and practices (r = .76, p<0.001) was observed. Microbiological tests confirmed the presence of Escherichia coli and Enterococcus species by biochemical tests. Escherichia coli resistance to ampicillin was high (82.2%) and this was followed by trimethoprim-sulfamethoxazole (75.8%), gentamicin (22.5%), cefotaxime (13.1%), ciprofloxacin (7.9%), and tigecycline (0.5%). In terms of Enterococcus species, resistance to vancomycin, ampicillin and ciprofloxacin were at 79.7%, 50% and 23.4%, respectively. No resistance to tigecycline was observed in all the Enterococcus isolates. The study also found that 26.7% (n=51) and 10.1% (n=16) of the E. coli and Enterococcus isolates, respectively, were resistant to more than one class of antibiotics. The study revealed a high risk of cross-contamination with resistant pathogens. Therefore, good hygienic practices in the farm-to-fork continuum, enforcement of food safety regulations and capacity building are pivotal in reducing the dissemination of resistant genes in the pork chain. The importance of antimicrobial stewardship should not be underemphasized if optimal utilisation of antimicrobials is to be attained. Belgian Directorate-General for Development Cooperation (DGD) within the DGD-ITM Framework Agreement 5 Veterinary Tropical Diseases MSc (Global One Health) Unrestricted Faculty of Veterinary Science SDG-03: Good health and well-being 2025-04-03T07:31:07Z 2025-04-03T07:31:07Z 2025-09 2025-03 Mini Dissertation * S2025 http://hdl.handle.net/2263/101861 DOI: https://doi.org/10.25403/UPresearchdata.28705328.v1 https://doi.org/10.25403/UPresearchdata.28705328 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Escherichia coli Enterococcus Food safety Knowledge, attitudes and practices (KAP) Northern Malawi Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers |
| title | Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers |
| title_full | Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers |
| title_fullStr | Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers |
| title_full_unstemmed | Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers |
| title_short | Prevalence of antimicrobial-resistant Escherichia coli and Enterococcus species in pigs in northern Malawi, and the knowledge, attitudes and practices (KAP) amongst butchers |
| title_sort | prevalence of antimicrobial resistant escherichia coli and enterococcus species in pigs in northern malawi and the knowledge attitudes and practices kap amongst butchers |
| topic | UCTD Sustainable Development Goals (SDGs) Escherichia coli Enterococcus Food safety Knowledge, attitudes and practices (KAP) Northern Malawi |
| url | http://hdl.handle.net/2263/101861 https://doi.org/10.25403/UPresearchdata.28705328 |