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The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers

Dissertation (MSc Agric (Animal Science))--University of Pretoria, 2025.

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Other Authors: Nkuwana , Thobela
Format: Thesis
Language:English
Published: University of Pretoria 2025
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access_status_str Open Access
author2 Nkuwana , Thobela
author_browse Nkuwana , Thobela
author_facet Nkuwana , Thobela
collection Thesis
dc_rights_str_mv © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc Agric (Animal Science))--University of Pretoria, 2025.
format Thesis
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institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:37:50.718Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/103683 The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers Nkuwana , Thobela u19015692@tuks.co.za Plumstead, Peter Fourie, Jamie Leigh UCTD Sustainable Development Goals (SDGs) Phyatse Limestone Broiler Acid-binding capacity (ABC) Feed formulations Proximal gastrointestinal tract Dissertation (MSc Agric (Animal Science))--University of Pretoria, 2025. Limestone is a vital dietary ingredient in broiler diets and is the main source of Ca utilised in global feed formulations. However, it exhibits considerable variation in multiple physical and chemical characteristics. This study sought to quantify the variability of acid-binding capacity (ABC) in limestone, as well as the intrinsic limestone properties that may affect ABC. Furthermore, it aimed to investigate the relationship between limestone ABC and Ca and P digestibility. The trial utilised 40 different limestone samples that were measured for ABC at various timepoints, which were correlated with the various physical and chemical characteristics of the limestones. The in vivo trial used a 6 x 2 factorial design, consisting of 5 limestones of graded ABC values and diets with no added limestone. These diets were fed to broilers in the presence and absence of 1,000 FTU/kg phytase supplementation. A total of 648 broilers were allocated to one of 12 treatments, following a complete randomised block design. Birds were fed the treatment diets for 36 hours prior to being euthanised, and sampled for pH of the gizzard and proventriculus, as well as for ileal digesta. All data were captured and analysed using JMP 16.0 (JMP, 2022), using a full factorial analysis with block included as a random effect. Correlations were determined using Pearson correlation analysis, with significance set at P < 0.05. Correlation analysis reported the strongest correlations between GMD and ABC at 120 minutes (-0.43), and between 5-minute solubility and ABC at 120 minutes (0.48). Results from the in vivo study showed no effect of limestone ABC on the pH of the gizzard or proventriculus, but did report a main effect (P < 0.05) of phytase on gizzard pH. For digestibility, effects were reported for limestone ABC and phytase supplementation on the apparent ileal digestibility (AID) of Ca and P. Furthermore, an interaction was noted between limestone ABC and phytase supplementation on digestible P (dP). However, both Ca and P digestibility followed no clear response to limestone ABC, and one may hypothesise that the results may be more reflective of the limestone solubility profiles. Neuro Livestock Research Animal and Wildlife Sciences MSc Agric (Animal Science) Unrestricted Faculty of Natural and Agricultural Sciences SDG-02: Zero Hunger 2025-07-30T04:49:09Z 2025-07-30T04:49:09Z 2025-09 2025-06 Dissertation * S2025 http://hdl.handle.net/2263/103683 https://doi.org/10.25403/UPresearchdata.29666198 en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Sustainable Development Goals (SDGs)
Phyatse
Limestone
Broiler
Acid-binding capacity (ABC)
Feed formulations
Proximal gastrointestinal tract
The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers
title The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers
title_full The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers
title_fullStr The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers
title_full_unstemmed The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers
title_short The determination of acid-binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract pH, calcium and phosphorous digestibility in broilers
title_sort determination of acid binding capacity of various limestones and subsequent impact on proximal gastrointestinal tract ph calcium and phosphorous digestibility in broilers
topic UCTD
Sustainable Development Goals (SDGs)
Phyatse
Limestone
Broiler
Acid-binding capacity (ABC)
Feed formulations
Proximal gastrointestinal tract
url http://hdl.handle.net/2263/103683
https://doi.org/10.25403/UPresearchdata.29666198