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Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2022.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2025
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| _version_ | 1867613639908786176 |
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| access_status_str | Open Access |
| author2 | Karama, Musafiri |
| author_browse | Karama, Musafiri |
| author_facet | Karama, Musafiri |
| collection | Thesis |
| dc_rights_str_mv | © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/104244 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:20.633Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/104244 Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa Karama, Musafiri u28556021@tuks.co.za Marufu, Munyaradzi Christopher Malahlela, Mogaugedi Nancy UCTD Goats Serotypes Virulence South Africa Veterinary science theses SDG-02 Veterinary science theses SDG-06 Veterinary science theses SDG-12 Dissertation (MSc (Veterinary Science))--University of Pretoria, 2022. Shiga-toxin-producing Escherichia coli is a foodborne pathogen commonly associated with human disease characterized by mild or bloody diarrhoea hemorrhagic colitis and hemolytic uremic syndrome. This study investigated the occurrence of STEC in fecal samples of 289 goats in South Africa using microbiological culture and PCR. Furthermore, 628 goat STEC isolates were characterized by serotype (O:H) and major virulence factors by PCR. STEC was found in 80.2% (232/289) of goat fecal samples. Serotyping of 628 STEC isolates revealed 63 distinct serotypes including four of the major top seven STEC serogroups which were detected in 12.1% (35/289) of goats: O157:H7, 2.7% (8/289); O157:H8, 0.3%, (1/289); O157:H29, 0.3% (1/289); O103:H8, 7.6% (22/289); O103:H56, 0.3% (1/289); O26:H2, 0.3% (1/289); O111:H8, 0.3% (1/289) and 59 non-O157 STEC serotypes. Twenty-four of the sixtythree serotypes were previously associated with human disease. Virulence genes were distributed as follows: stx1, 60.6% (381/628); stx2, 72.7% (457/628); eaeA, 22.1% (139/628) and hlyA, 78.0% (490/628). Both stx1 and stx2 were found in 33.4% (210/628) of isolates. In conclusion, goats in South Africa are a reservoir and potential source of diverse STEC serotypes that are potentially virulent for humans. Further molecular characterization will be needed to fully assess the virulence potential of goat STEC isolates and their capacity to cause disease in humans. es2026 Paraclinical Sciences MSc (Veterinary Science) Unrestricted Faculty of Veterinary Science SDG-02: Zero hunger SDG-06: Clean water and sanitation SDG-12: Responsible consumption and production 2025-09-08T08:34:08Z 2025-09-08T08:34:08Z 2023-04 2022-05 Dissertation * A2023 http://hdl.handle.net/2263/104244 N/A en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Goats Serotypes Virulence South Africa Veterinary science theses SDG-02 Veterinary science theses SDG-06 Veterinary science theses SDG-12 Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa |
| title | Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa |
| title_full | Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa |
| title_fullStr | Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa |
| title_full_unstemmed | Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa |
| title_short | Occurrence and characterization of Shiga toxin-producing Escherichia coli from goats on communal rangeland in Gauteng South Africa |
| title_sort | occurrence and characterization of shiga toxin producing escherichia coli from goats on communal rangeland in gauteng south africa |
| topic | UCTD Goats Serotypes Virulence South Africa Veterinary science theses SDG-02 Veterinary science theses SDG-06 Veterinary science theses SDG-12 |
| url | http://hdl.handle.net/2263/104244 |