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Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros

Thesis (PhD (Veterinary Sciences))--University of Pretoria, 2018.

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Other Authors: Thompson, P.N. (Peter N.)
Format: Thesis
Language:English
Published: University of Pretoria 2025
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access_status_str Open Access
author2 Thompson, P.N. (Peter N.)
author_browse Thompson, P.N. (Peter N.)
author_facet Thompson, P.N. (Peter N.)
collection Thesis
dc_rights_str_mv © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Thesis (PhD (Veterinary Sciences))--University of Pretoria, 2018.
format Thesis
id oai:repository.up.ac.za:2263/107111
institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:38:12.930Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher University of Pretoria
publisherStr University of Pretoria
record_format dspace
source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/107111 Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros Thompson, P.N. (Peter N.) cindy.harper@up.ac.za O'Brien, Stephen J. Harper, Cindy Kim UCTD Sustainable Development Goals (SDGs) Forensic Poaching Thesis (PhD (Veterinary Sciences))--University of Pretoria, 2018. Rhinoceros horn is now worth more, per unit weight, than gold, diamonds, or cocaine. Rhinoceros horn has been used in traditional Asian medicine as a presumed cure for a wide range of ailments. Rhinoceros poaching in South Africa has, on average, more than doubled each year over the past 5 years with the rapid economic growth in east and southeast Asia being assumed to be the primary factor driving the increased demand for horn. Here we report on the characterization of methods for genomic DNA extraction from rhinoceros horn and on DNA profiling systems for white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros. The DNA profiling system described includes 22 short tandem repeat (STR), or microsatellite, markers and a gender marker (ZF1), which have been used previously in various studies on rhinoceros. Using a u value of 0.1, a conservative estimate of random match probability in 5 white rhinoceros ranged from 1:7.3 x 106 to 1:3.0 x 108. Given that the total population of white rhinoceros is approximately 20,000 such random match probabilities indicate that the genotyping system described provides data which can be used for evidentiary purposes. Furthermore, the methods are appropriate for use in investigations involving trace amounts of rhinoceros horn and the matching of profiles obtained from seized rhinoceros horn with material collected from live animals or poached carcasses. Production Animal Studies PhD (Veterinary Sciences) Unrestricted Faculty of Veterinary Science SDG-03: Good health and well-being SDG-15: Life on land 2025-12-08T07:13:16Z 2025-12-08T07:13:16Z 2019 2018-10 Thesis * A2019 http://hdl.handle.net/2263/107111 en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Sustainable Development Goals (SDGs)
Forensic
Poaching
Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros
title Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros
title_full Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros
title_fullStr Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros
title_full_unstemmed Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros
title_short Development and implementation of the Rhinoceros DNA Index System (RhODIS®) for the forensic analysis and biological management of African rhinoceros
title_sort development and implementation of the rhinoceros dna index system rhodis r for the forensic analysis and biological management of african rhinoceros
topic UCTD
Sustainable Development Goals (SDGs)
Forensic
Poaching
url http://hdl.handle.net/2263/107111