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De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2026
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| _version_ | 1867613455015477248 |
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| access_status_str | Open Access |
| author2 | Berger, David Kenneth |
| author_browse | Berger, David Kenneth |
| author_facet | Berger, David Kenneth |
| collection | Thesis |
| dc_rights_str_mv | © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/107659 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:24.683Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/107659 De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery Berger, David Kenneth dave.berger@up.ac.za Pierneef, Rian Ewald Welgemoed , Tanya UCTD RNA-Sequence Cercospora zeina De novo assembly Zea mags Grey leaf spot Dissertation (MSc)--University of Pretoria, 2018. Maize is an economically important crop world-wide. This is especially true in South Africa where it is a staple crop and production contributes to food security. A major threat to maize production is a fungal foliar disease, grey leaf spot, that in South Africa is caused by Cercospora zeina. Controlling this disease to prevent the current yield losses is therefore important for food security. Ideally genetically resistant crops would be used, but there are only a few known defence genes against this disease. This study addressed this gap by developing a de novo assembly pipeline to identify maize genes involved in quantitative resistance against C. zeina. The study was done on three C. zeina-challenged and three control samples from a B73 maize line that had been introgressed with a quantitative trait locus for resistance against the disease. It was hypothesised that there would be a novel gene present in the introgressed QTL region that conferred the resistance against C. zeina infection. The research presented in this dissertation is based on bioinformatics and experimental work conducted in the Molecular Plant-Pathogen Interactions (MPPI) group at the University of Pretoria, South Africa. The dissertation is presented in the form of six chapters. FUNDING : The financial assistance of the National Research Foundation (NRF) towards this research is hereby acknowledged. Biochemistry, Genetics and Microbiology (BGM) MSc (Bioinformatics) Restricted Faculty of Natural and Agricultural Sciences SDG-02: Zero Hunger 2026-01-28T09:08:51Z 2026-01-28T09:08:51Z 2019-04-24 2018-11-20 Dissertation * A2019 http://hdl.handle.net/2263/107659 N/A en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD RNA-Sequence Cercospora zeina De novo assembly Zea mags Grey leaf spot De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery |
| title | De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery |
| title_full | De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery |
| title_fullStr | De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery |
| title_full_unstemmed | De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery |
| title_short | De novo assembly of transcriptomes from near-isogenic maize lines for novel defence gene discovery |
| title_sort | de novo assembly of transcriptomes from near isogenic maize lines for novel defence gene discovery |
| topic | UCTD RNA-Sequence Cercospora zeina De novo assembly Zea mags Grey leaf spot |
| url | http://hdl.handle.net/2263/107659 |