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Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin
Dissertation (MSc)--University of Pretoria, 2018.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2026
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| _version_ | 1867613536701644800 |
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| access_status_str | Open Access |
| author2 | Van Heerden, Henriette |
| author_browse | Van Heerden, Henriette |
| author_facet | Van Heerden, Henriette |
| collection | Thesis |
| dc_rights_str_mv | © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2018. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/107673 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:42.843Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/107673 Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin Van Heerden, Henriette henriette.vanheerden@up.ac.za Munyeme, Musso Mwamba UCTD Bacillus anthracis Anthrax Zambesi basin MLVA VNTR Dissertation (MSc)--University of Pretoria, 2018. Outbreaks of anthrax in livestock and wildlife have become a seasonal occurrence in Zambia. Consequently, the disease caused by Bacillus anthracis, has also been shown to be a classical zoonoses as almost with every outbreak, there is human involvement, where they get infected through contact and ingestion of contaminated meat/products. Accordingly, the disease has a direct effect on the livelihoods of the people living in the known endemic areas through which the socio-anthropogenic as well as socioeconomic determinants of the disease are observed. This scenario has been observed to occur almost annually in two ecosystems along the Zambezi basin, that is the Upper and Lower Zambezi basins. Thus, the aim of this study was to characterize and determine the genetic diversity of B. anthracis causing these mortalities in animals and humans in the two different sites of the Zambezi basin through molecular characterization. The first samples were collected from four (4) districts, located in the Upper Zambezi Basin, of Western Province of Zambia where livestock, particularly cattle, and human beings were infected following outbreaks in 2000, 2013 through to 2016. The second collection was from outbreaks in the Lower Zambezi basin during 2012, where predominantly wildlife was infected with concurrent outbreaks in human populations. Using the agarose gel methodology, multiple-locus variable-number of tandem repeat analysis (MLVA) based on 11 markers indicated 3 genotypes amongst 28 B. anthracis strains from Zambia in this study with one dominant genotype i.e. the Ancient A (Aβ) sublineage of the A -clade (consisting of 85.7% (24/28)). This indicate clonality of B. anthracis strains in this study from Zambia circulating in livestock, wildlife, humans and surrounding environments in the two regions. FUNDING : I am sincerely grateful to FA3 III-N02000 = Belgian Directorate General for Development Co-operation Framework Agreement (3-III DGD/ITM 2014-2016) awarded to the Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria through which I received the financial support for me to pursue this program. Veterinary Tropical Diseases MSc (Veterinary Science) Restricted Faculty of Veterinary Science SDG-03: Good health and well-being 2026-01-28T09:16:54Z 2026-01-28T09:16:54Z 2018-12 2018-12 Dissertation * A2019 http://hdl.handle.net/2263/107673 N/A en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Bacillus anthracis Anthrax Zambesi basin MLVA VNTR Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin |
| title | Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin |
| title_full | Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin |
| title_fullStr | Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin |
| title_full_unstemmed | Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin |
| title_short | Molecular characterisation of Bacillus anthracis involved in outbreaks in the Zambezi basin |
| title_sort | molecular characterisation of bacillus anthracis involved in outbreaks in the zambezi basin |
| topic | UCTD Bacillus anthracis Anthrax Zambesi basin MLVA VNTR |
| url | http://hdl.handle.net/2263/107673 |