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Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus
Dissertation (MSc (Microbiology and Plant Pathology))--University of Pretoria, 2015.
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| Format: | Thesis |
| Language: | English |
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2026
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| _version_ | 1867613623953653760 |
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| access_status_str | Open Access |
| author2 | Coutinho, Teresa A. |
| author_browse | Coutinho, Teresa A. |
| author_facet | Coutinho, Teresa A. |
| collection | Thesis |
| description | Dissertation (MSc (Microbiology and Plant Pathology))--University of Pretoria, 2015. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/110146 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:05.758Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/110146 Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus Coutinho, Teresa A. kay.bophela@fabi.up.ac.za Venter, Stephanus N. Bophela, Khumbuzile Nokwanda Enterobacter MISA Eucalyptus Dissertation (MSc (Microbiology and Plant Pathology))--University of Pretoria, 2015. Bacterial dieback was first reported in Australia in 1974 from Corymbria citriodora. The causal agent was described as Xanthomonas campestris pv. eucalypti (= emended X. dyei subsp. eucalypti). Subsequent records of bacterial blight and dieback were reported in South Africa and Brazil and the causal agents were Pantoea ananatis and Xanthomonas axonopodis, respectively. In recent studies strains of Enterobacter and related genera were reported to have an association with blight of Eucalyptus. However, only a few records exist in the literature of Enterobacter species causing disease in woody plants. Most species in this genus are known as causal agents of nosocomial and community-acquired infections of humans. In a recent study, Enterobacter isolates were obtained together with Pantoea spp. from diseased and healthy stem, roots and leaves of Eucalyptus seedlings showing blight symptoms. In addition, strains of Xanthomonas were obtained from infected Eucalyptus leaves in three regions, i.e. Uruguay, Indonesia and South Africa. Therefore, the aim of this study was to identify a collection of isolates from Enterobacteriaceae and Xanthomonas previously obtained from diseased Eucalyptus seedlings and cuttings, and determine whether or not they are pathogens of this host. Identifications were based on a multilocus sequence analysis (MLSA) approach based on housekeeping genes and the 16S rRNA gene was added for comparison and genus level identification. Based on the MLSA results iii of three housekeeping genes, i.e. atpD, gyrB and infB, three previously described species in Enterobacter, E. mori, E. soli and E. asburiae, and eight potentially novel species (MLSA groups A-H) from two genera, Enterobacter and Kosakonia, within the Enterobacteriaceae, were identified. Likewise, the MLSA results, based on four housekeeping genes, dnaK, fyuA, gyrB and rpoD, revealed three previously described species in Xathomonas, namely X. vasicola, X. perforans and X. fuscans. Isolates from all species identified in this study were able to induce typical blight symptoms in cuttings of a susceptible Eucalyptus grandis/nitens hybrid. These results will impact on the future production of this and other susceptible clones in forest nurseries. Microbiology and Plant Pathology MSc (Microbiology and Plant Pathology) 2026-05-15T17:26:26Z 2026-05-15T17:26:26Z 15/11/16 2015 Dissertation http://hdl.handle.net/2263/110146 en application/pdf |
| spellingShingle | Enterobacter MISA Eucalyptus Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus |
| title | Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus |
| title_full | Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus |
| title_fullStr | Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus |
| title_full_unstemmed | Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus |
| title_short | Enterobacter and Xanthomonas species associated with blight and die-back of Eucalyptus |
| title_sort | enterobacter and xanthomonas species associated with blight and die back of eucalyptus |
| topic | Enterobacter MISA Eucalyptus |
| url | http://hdl.handle.net/2263/110146 |