Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis
Dissertation (MSc (Human Physiology))--University of Pretoria, 2017.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2026
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613585317822464 |
|---|---|
| access_status_str | Open Access |
| author2 | Van den Bout, Jan Iman |
| author_browse | Van den Bout, Jan Iman |
| author_facet | Van den Bout, Jan Iman |
| collection | Thesis |
| description | Dissertation (MSc (Human Physiology))--University of Pretoria, 2017. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/110153 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:29.059Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/110153 Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis Van den Bout, Jan Iman botesmandie@gmail.com Joubert, Annie Botes, Mandie EE-IS-One Breast cancer Cell detachment Anoikis 2-Methoxyestradiol Dissertation (MSc (Human Physiology))--University of Pretoria, 2017. 2-Methoxy estradiol (2ME2) is a natural metabolite of oestrogen that has been shown to exert antiproliferative effects on many cancer cell lines but lacks biological function due to rapid metabolism. Several studies suggest that 2ME2 impedes cell cycle progression although an exact molecular mechanism remains elusive. In this report, the characteristics of a nonsulphamoylated 2ME2 derivative (EE-15-one) are described as it, unlike 2ME2, induces rapid, and irreversible cell detachment in both oestrogen receptor positive and triple negative breast cancer cell lines leading to the induction of anoikis. It does so by causing the disassembly of focal adhesions and the loss of the actin stress fibers while at the same time reducing focal adhesion kinase activity. Extracellular regulated kinase (ERK) activation was also increased and sustained similarly to effects reported for 17_-oestradiol. Therefore, EE15-one is a novel oestrone derivative that induces cancer cell anoikis possibly through the inactivation of integrins and the dissolution of the actin cytoskeleton independently of the oestrogen receptor status of the cell. Physiology MSc (Human Physiology) 2026-05-15T17:26:28Z 2026-05-15T17:26:28Z 18/02/12 2017 Dissertation http://hdl.handle.net/2263/110153 en application/pdf |
| spellingShingle | EE-IS-One Breast cancer Cell detachment Anoikis 2-Methoxyestradiol Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis |
| title | Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis |
| title_full | Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis |
| title_fullStr | Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis |
| title_full_unstemmed | Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis |
| title_short | Identification of the target of EE-15-one and elucidation of the pathway leading from EE-15-one exposure to anoikis |
| title_sort | identification of the target of ee 15 one and elucidation of the pathway leading from ee 15 one exposure to anoikis |
| topic | EE-IS-One Breast cancer Cell detachment Anoikis 2-Methoxyestradiol |
| url | http://hdl.handle.net/2263/110153 |