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The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe
Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2016.
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| Format: | Thesis |
| Language: | English |
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2026
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| _version_ | 1867613549539360768 |
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| access_status_str | Open Access |
| author2 | Van Heerden, Henriette |
| author_browse | Van Heerden, Henriette |
| author_facet | Van Heerden, Henriette |
| collection | Thesis |
| description | Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/110171 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:55.093Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/110171 The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe Van Heerden, Henriette jvburumu@yahoo.com Gomo, Calvin Burumu, Joan Varaidzo Zimbabwe PCR Polymononuclear cells Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2016. Brucellosis is an important zoonotic disease caused by the genus Brucella. Bovine brucellosis caused by B. abortus is endemic in sub-Saharan African countries, which include Zimbabwe. Reliability of test methods used for detection of bovine brucellosis is crucial for control and eradication. A combination of culturing, serological test(s) and/or PCR is used to confirm brucellosis since no single test absolutely identifies Brucella. Serology is primarily used for bovine brucellosis diagnosis. Culture and identification is the gold standard for Brucella diagnosis. PCR has been suggested as a diagnostic method but also has sensitivity problems since the stage of infection influences the number and location of Brucella organisms in white blood cells and lymphoid tissue glands. During infection, Brucella is absorbed by macrophages, where the bacterium survives and multiplies within its host. Therefore in this study we investigated the use of peripheral blood mononuclear cells (PBMCs) for the detection of Brucella DNA using the ITS 279 and ITS66 primers that target Brucella specific 16S-23S rRNA interspacer region and B. abortus species specific (BaSS) PCR that targets insertion sequence IS711 downstream of the alkB gene. Samples from 35 cows with known and ongoing bovine brucellosis infection were collected from Esigodini district in Zimbabwe and tested for bovine brucellosis using the Rose Bengal test (RBT) and complement fixation test (CFT). Only cows (n=26) with both serological tests and PCR results were used in analyses. Brucella antibodies were detected with the RBT in 65% (17 of the 26) cows tested and all but one animal of the RBT seropositive animals also tested positive using CFT with titres ranging from 32 to 128. PBMCs from 26 animals, 16 seropositive and 10 seronegative, were tested using the 16S-23S rRNA interspacer region and BaSS PCR. Brucella DNA was detected in 96% of these animals using 16S-23S rRNA interspacer region PCR, with only one seronegative animal giving a negative result, whereas the B. abortus specific insertion sequence IS711 region was detected in 12% (3 of 26) of the 5 cows. The results from this study confirm the presence of bovine brucellosis in the villages in the Esigodini district in Zimbabwe. The results in this study suggest that the multi-copy 16S-23S rRNA interspacer PCR using PBMCs for the detection of Brucella DNA could be used for diagnosis in brucellosis positive herds, but need to be further investigated. The less sensitive single copy BaSS PCR detecting the insertion sequence IS711 downstream of the alkB gene confirmed that 3 brucellosis seropositive animals were infected with B. abortus. It is of the utmost importance to implement control measures and raise publish awareness amongst the villagers on zoonotic transmission of brucellosis. Veterinary Tropical Diseases MSc (Veterinary Tropical Diseases) 2026-05-15T17:26:33Z 2026-05-15T17:26:33Z 16/02/08 2016 Dissertation http://hdl.handle.net/2263/110171 en application/pdf |
| spellingShingle | Zimbabwe PCR Polymononuclear cells The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe |
| title | The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe |
| title_full | The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe |
| title_fullStr | The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe |
| title_full_unstemmed | The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe |
| title_short | The use of peripheral blood mononuclear cells for detection of brucella using PCR in seropositive cows in Zimbabwe |
| title_sort | use of peripheral blood mononuclear cells for detection of brucella using pcr in seropositive cows in zimbabwe |
| topic | Zimbabwe PCR Polymononuclear cells |
| url | http://hdl.handle.net/2263/110171 |