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Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2016.
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| Format: | Thesis |
| Language: | English |
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2026
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| _version_ | 1867613482476634112 |
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| access_status_str | Open Access |
| author2 | Michel, Anita Luise |
| author_browse | Michel, Anita Luise |
| author_facet | Michel, Anita Luise |
| collection | Thesis |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2016. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/110186 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:51.203Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/110186 Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates Michel, Anita Luise jchileshe06@yahoo.com Rutten, V. Chileshe, Josephine Bovine tuberculosis Mycobacterium bovis Control measures Vaccines Immune responses Dissertation (MSc (Veterinary Science))--University of Pretoria, 2016. The aim of this study was to evaluate and compare the cell-mediated and humoral immune responses elicited in cattle by three different vaccine formulations (live M. bovis BCG, formalin inactivated M. bovis BCG and heat killed M. bovis). A total of 24 calves were used in this experiment, three out of four treatment groups of 6 calves each were vaccinated by the subcutaneous route with one of the three vaccines while one group of 6 calves served as control. Animals were subjected to the tuberculin skin test and sampled at prescribed times to assess host systemic immunity in terms of interferon gamma (IFN-_) response, using Bovigam® and antibody levels using an IDEXX® M. bovis antibody test and compared between the four treatment groups. Results show that subcutaneous vaccination with killed whole M. bovis cells and live BCG elicited detectable cellular and humoral immune responses in cattle. While the killed BCG vaccine formulation only induced cell mediated immunity. The treatment group which received the heat killed M. bovis vaccine showed the most significant increase in the IFN-_ ix responses directed against bovine tuberculin (PPD-B) from 4 weeks post vaccination to the end of the experiment. Bovine TB-specific humoral responses represented by IgG antibodies were not detected in the group vaccinated with formalin inactivated M. bovis BCG. The groups vaccinated with live M. bovis BCG had detectable but low levels of antibody responses and heat killed M. bovis had high levels of IgG antibodies, respectively. The latter indicated stronger host immune responses in the heat killed M. bovis group as compared to the live M. bovis BCG vaccine and the formalin inactivated M. bovis BCG vaccine groups. In conclusion, levels of immune responses detected indicate a superior cell-mediated and humoral immune response of the heat killed M. bovis group, which makes it a suitable vaccine candidate for field trials. In addition, the advantages of the use of heat killed M. bovis vaccine as compared to a live M. bovis BCG vaccine formulation are i) the killed M. bovis vaccine would not shed into the environment and ii) the potential health risk for immunecompromised individuals of live BCG is ruled out. Veterinary Tropical Diseases MSc (Veterinary Science) 2026-05-15T17:26:36Z 2026-05-15T17:26:36Z 16/11/29 2016 Dissertation http://hdl.handle.net/2263/110186 en application/pdf |
| spellingShingle | Bovine tuberculosis Mycobacterium bovis Control measures Vaccines Immune responses Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| title | Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| title_full | Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| title_fullStr | Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| title_full_unstemmed | Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| title_short | Host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| title_sort | host immune response profiles of calves following vaccination with live and inactivated mycobacterium bovis vaccine candidates |
| topic | Bovine tuberculosis Mycobacterium bovis Control measures Vaccines Immune responses |
| url | http://hdl.handle.net/2263/110186 |