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The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis)
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2003.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613652980334592 |
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| access_status_str | Open Access |
| author2 | Rutten, Victor P.M.G. |
| author_browse | Rutten, Victor P.M.G. |
| author_facet | Rutten, Victor P.M.G. |
| collection | Thesis |
| dc_rights_str_mv | © 2003, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2003. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/23032 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:33.692Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/23032 The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) Rutten, Victor P.M.G. Venter, Estelle Hildegard Tijhaar, Edwin Michel, Anita Luise upetd@ais.up.ac.za Morar, Darshana No key words available UCTD Dissertation (MSc (Veterinary Science))--University of Pretoria, 2003. The objective of this project was to design tools for a diagnostic test that will prove valuable in the detection of tuberculosis in elephants and rhinoceros by using the cytokine IFNγ as an indicator of Mycobacterium bovis responsiveness. Interferon-gamma (IFNγ), a type II interferon, is a cytokine mainly produced by Th1 and cytotoxic T-cells expressing surface markers CD4 and CD8, respectively and natural killer cells (Ibelgaufts; 1999). In response to a mycobacterial infection antigen specific Th1- and cytotoxic T-cells are induced. When these cells encounter their specific mycobacterial antigen, they will respond by producing IFNγ. Based on this principle a diagnostic test was developed. In this test PBMCs will be stimulated with M.bovis specific antigen and the subsequent production of IFNγ by specific T-helper cells will be determined by IFNγ of elephants and rhinoceros. In order to develop such an assay recombinant elephant and rhinoceros IFNγ was cloned, sequenced, expressed, purified and subsequently a monoclonal antibody against IFNγ was produced. Monoclonal antibodies were selected by a number of ELISAs using recombinant IFNγ. Preliminary results are promising and further tests are underway regarding the specificity and sensitivity of the assay before field trials can be performed. The results of this study has significant implications in the design of an IFNγ diagnostic kit for the diagnosis of tuberculosis, as caused by M.bovis, in elephants and rhinoceros as well as other wildlife affected by this debilitating disease. Veterinary Tropical Diseases unrestricted 2013-09-06T14:23:08Z 2005-03-09 2013-09-06T14:23:08Z 2003-09-10 2006-03-09 2005-03-09 Dissertation Morar, D 2003, The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis), MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/23032 > http://hdl.handle.net/2263/23032 http://upetd.up.ac.za/thesis/available/etd-03092005-080343/ © 2003, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | No key words available UCTD The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) |
| title | The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) |
| title_full | The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) |
| title_fullStr | The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) |
| title_full_unstemmed | The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) |
| title_short | The development of an interferon-gamma (IFNγ) assay for the diagnosis of tuberculosis in African elephants (Loxodonta africana) and black rhinoceros (Diceros bicornis) |
| title_sort | development of an interferon gamma ifnγ assay for the diagnosis of tuberculosis in african elephants loxodonta africana and black rhinoceros diceros bicornis |
| topic | No key words available UCTD |
| url | http://hdl.handle.net/2263/23032 http://upetd.up.ac.za/thesis/available/etd-03092005-080343/ |