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The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa
Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2001.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613617707286528 |
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| access_status_str | Open Access |
| author2 | Van Vuuren, Moritz |
| author_browse | Van Vuuren, Moritz |
| author_facet | Van Vuuren, Moritz |
| collection | Thesis |
| dc_rights_str_mv | © 2001, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2001. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/23551 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:00.149Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/23551 The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa Van Vuuren, Moritz upetd@ais.up.ac.za Kabongo, Prudence Ngalula No key words available UCTD Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2001. A limited number of scientific publications dealing with aspects of BVDV infection have emanated from southern Africa. This study describes the isolation of BVD viruses, gene sequence analysis of the 5' non-translated region (5' NTR) of the genome, the generation of phylogenetic data of local strains and the recording of clinical signs associated with each isolate. Specimens (n=352) collected during 1998-1999, from live and dead animals from different farming systems, were obtained from private practitioners, feedlot consultants and abattoirs throughout the country. Specimens from buffaloes (cerus caffer the Kruger National Park were included as specimens from dead animals. Three cell lines and 200 tubes of pooled foetal bovine sera were also processed. Standard cell culture techniques to isolate virus were followed. Techniques designed to detect BVDV antigen or nucleic acid such as antigen capture enzyme-linked immunosorbent assay and polymerase chain reaction, were used on blood, organs and cell lines. The indirect fluorescent antibody test was used for antibody detection. Twenty-five isolates from cattle were confirmed as BVDV with PCR and after analysis of the 5'NTR, the most conserved part of the genome, a phylogenetic tree was constructed. All strains were noncytopathic and were identified as BVDV I, either BVDV Ia (NADL-like) or BVDV Ic or BVDV I* subgroups. BVDV was not detected in 37 lymph nodes obtained from 37 buffaloes in the Kruger National Park. Of the clinical signs in cattle from which virus was isolated, pyrexia and respiratory distress was the most frequent (46,7%), followed by pyrexia and diarrhoea (20%), respiratory disease without pyrexia (20%) and diarrhoea without pyrexia (13,3%). Abortion, congenital malformations, haemorrhagic syndrome and poor growth were also included as criteria for selection of animals for specimen collection. Veterinary Tropical Diseases unrestricted 2013-09-06T15:33:32Z 2005-03-29 2013-09-06T15:33:32Z 2001-08-01 2006-03-29 2005-03-29 Dissertation Kabongo, P 2001, The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/23551 > http://hdl.handle.net/2263/23551 http://upetd.up.ac.za/thesis/available/etd-03292005-121311/ © 2001, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | No key words available UCTD The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa |
| title | The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa |
| title_full | The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa |
| title_fullStr | The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa |
| title_full_unstemmed | The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa |
| title_short | The isolation and characterization of bovine viral diarrhoea viruses from cattle in South Africa |
| title_sort | isolation and characterization of bovine viral diarrhoea viruses from cattle in south africa |
| topic | No key words available UCTD |
| url | http://hdl.handle.net/2263/23551 http://upetd.up.ac.za/thesis/available/etd-03292005-121311/ |