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Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2001.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613561958694912 |
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| access_status_str | Open Access |
| author2 | Van Vuuren, Moritz |
| author_browse | Van Vuuren, Moritz |
| author_facet | Van Vuuren, Moritz |
| collection | Thesis |
| dc_rights_str_mv | © 2001, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2001. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24019 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:38:06.885Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24019 Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen Van Vuuren, Moritz Guthrie, Alan John Hamblin, Christopher upetd@ais.up.ac.za Crafford, Jan Ernst No key words available UCTD Dissertation (MSc (Veterinary Science))--University of Pretoria, 2001. The main purpose of this work was to develop rapid and reliable techniques that will prove valuable in epidemiological studies of equine encephalosis virus (EEV), the detection and identification of the virus for the laboratory confirmation of the clinical diagnosis and for the differential diagnosis between EEV and African horsesickness virus (AHSV). Two enzyme linked immunosorbent assays (ELISA) were developed. A polyclonal antibody-based, group-specific, indirect sandwich ELISA for the detection of EEV antigen was developed. The design of the assay was based on the methods currently used for the detection of AHSV. The cut-off value (absorbance of 0.15) was determined using populations of known negative specimens. No cross-reactions were recorded with viruses from other orbivirus serogroups or from other arboviruses. The assay proved to be sensitive and specific for the rapid detection of EEV and viral antigens in cell culture and mouse brain preparations. A polyclonal antibody-based, group-specific, competitive ELISA for the detection of antibodies to EEV was developed. No cross-reactions were recorded with the reference sera prepared against other orbivirus serogroups or other arboviruses. The cut-off (29.5% inhibition) value was determined using populations of known positive and negative sera. Analysis of the data showed the assay to be highly repeatable, sensitive and specific. Veterinary Tropical Diseases unrestricted 2013-09-06T16:23:42Z 2005-04-19 2013-09-06T16:23:42Z 2001-11-04 2006-04-19 2005-04-19 Dissertation Crafford, J 2001, Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24019 > http://hdl.handle.net/2263/24019 http://upetd.up.ac.za/thesis/available/etd-04192005-144818/ © 2001, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | No key words available UCTD Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| title | Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| title_full | Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| title_fullStr | Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| title_full_unstemmed | Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| title_short | Development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| title_sort | development and validation of enzyme linked immunosorbent assays for detection of equine encephalosis virus antibody and antigen |
| topic | No key words available UCTD |
| url | http://hdl.handle.net/2263/24019 http://upetd.up.ac.za/thesis/available/etd-04192005-144818/ |