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Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines
Dissertation (MSc (Genetics))--University of Pretoria, 2008.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613668134354944 |
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| access_status_str | Open Access |
| author2 | Oberholster, A.M. |
| author_browse | Oberholster, A.M. |
| author_facet | Oberholster, A.M. |
| collection | Thesis |
| dc_rights_str_mv | © University of Pretoria 20 |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2008. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24064 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:48.175Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24064 Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines Oberholster, A.M. charline.kamburona@up.ac.za Cilliers, A.M. Kamburona, Charline Kausana Putative transformants Peanut UCTD Dissertation (MSc (Genetics))--University of Pretoria, 2008. The first chapter gives an overall introduction to Arachis hypogaea L., the crop that was studied in this thesis. The literature reviewed touched on various aspects of the crop in general such as the importance of peanut as a crop, its botanical description, origin and distribution world-wide. Different marker studies done on A. hypogaea L. and their extents have also been reviewed. The chapter also looked at the oryzacystatin-1 (OC-1) gene as a possible candidate gene for peanut improvement in South Africa (Chapter I). Agronomic data was collected in an attempt to observe and evaluate variability amongst the 18 commercially cultivated peanut lines from South Africa. The growth habits, growth seasons, testa colours, total oil percentages and oleic and linoleic acid ratios were the agronomic characters used to study variation (Chapter II). One South African commercially cultivated peanut line, JL 24, was transformed with the oryzacystatin-1 (OC-1) gene, which was isolated from rice. Three to four week old plantlets were vacuum infiltrated with Agrobacterium during the transformation process and various methods were used to analyze the putative transformants (Chapter III). Two types of DNA markers along with phynotypic data were used to examine polymorphism among 18 South African peanut lines and a transformed line. The amplified fragment length polymorphisms (AFLPs) and simple sequences repeats (SSRs) were the two DNA markers used in this study to determine the level of variation amongst these lines (Chapter IV). Genetics unrestricted 2013-09-06T16:34:15Z 2008-07-08 2013-09-06T16:34:15Z 2007-09-06 2008-07-08 2008-04-22 Dissertation a 2007 http://hdl.handle.net/2263/24064 http://upetd.up.ac.za/thesis/available/etd-04222008-111945/ © University of Pretoria 20 application/pdf University of Pretoria |
| spellingShingle | Putative transformants Peanut UCTD Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines |
| title | Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines |
| title_full | Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines |
| title_fullStr | Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines |
| title_full_unstemmed | Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines |
| title_short | Evaluating the genetic diversity and performance of peanut (Arachis hypogaea L.) lines |
| title_sort | evaluating the genetic diversity and performance of peanut arachis hypogaea l lines |
| topic | Putative transformants Peanut UCTD |
| url | http://hdl.handle.net/2263/24064 http://upetd.up.ac.za/thesis/available/etd-04222008-111945/ |