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Identification of Penicillium species in the South African litchi export chain
Dissertation (MSc)--University of Pretoria, 2008.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613699881041920 |
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| access_status_str | Open Access |
| author2 | Korsten, Lise |
| author_browse | Korsten, Lise |
| author_facet | Korsten, Lise |
| collection | Thesis |
| dc_rights_str_mv | © 2008 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2008. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24242 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:18.073Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24242 Identification of Penicillium species in the South African litchi export chain Korsten, Lise upetd@up.ac.za Jacobs, Rene Johnston, Candice Leigh Morphological examination Secondary metabolites Biochemical analysis Penicillium South african litchi export UCTD Dissertation (MSc)--University of Pretoria, 2008. Penicillium species have been studied for over 200 years and the genus was first described by Link in 1809. Initially, morphological identification methods were used however, much diversity within the genus resulted in researchers seeking alternative techniques and approaches to improve accuracy. These methods involved biochemical analysis of secondary metabolites in conjunction with morphological examination. With the emergence of more accurate and rapid molecular identification tools, scientists embraced modem technology to address diversity challenges. In order to provide a more holistic approach towards the taxonomy of complex genera, morphological analysis remains an essential component in Penicillium identification. Penicillium species are omnipresent, dominant and problematic in postharvest environments. They are known to cause major losses in export markets due to fruit decay. The aim of this study was to identify species within the South African litchi export chain and develop a rapid method for Penicillium identification. This study used morphological as well as molecular identification methods in order to develop PCR-RFLP restriction maps for a number of dominant Penicillium species. Seventeen species of Penicillium were identified using conventional morphological methodology and DNA sequencing, both of which are laborious and time-consuming. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism provided reliability and repeatability as well as being a cost-effective and rapid identification alternative. A combined phylogenetic study indicated that the taxonomic position of several species may need to be reconsidered. Fourteen species were differentiated from one another through digestion of the â-tubulin gene region with five restriction enzymes. Banding patterns correlated well with phylogenetic and biochemical data of related studies, indicating that this method holds promise as a rapid identification procedure for Penicillium species. Microbiology and Plant Pathology unrestricted 2013-09-06T16:58:27Z 2009-04-30 2013-09-06T16:58:27Z 2008-07-11 2008 2009-04-30 Dissertation Johnston, CL 2008, Identification of Penicillium species in the South African litchi export chain, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24242 > C132/ag http://hdl.handle.net/2263/24242 http://upetd.up.ac.za/thesis/available/etd-04302009-144436/ © 2008 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Morphological examination Secondary metabolites Biochemical analysis Penicillium South african litchi export UCTD Identification of Penicillium species in the South African litchi export chain |
| title | Identification of Penicillium species in the South African litchi export chain |
| title_full | Identification of Penicillium species in the South African litchi export chain |
| title_fullStr | Identification of Penicillium species in the South African litchi export chain |
| title_full_unstemmed | Identification of Penicillium species in the South African litchi export chain |
| title_short | Identification of Penicillium species in the South African litchi export chain |
| title_sort | identification of penicillium species in the south african litchi export chain |
| topic | Morphological examination Secondary metabolites Biochemical analysis Penicillium South african litchi export UCTD |
| url | http://hdl.handle.net/2263/24242 http://upetd.up.ac.za/thesis/available/etd-04302009-144436/ |