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Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms
Dissertation (MSc(Microbiology))--University of Pretoria, 2006.
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University of Pretoria
2013
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| _version_ | 1867613693652500480 |
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| access_status_str | Open Access |
| author2 | Nel, Louis Hendrik |
| author_browse | Nel, Louis Hendrik |
| author_facet | Nel, Louis Hendrik |
| collection | Thesis |
| dc_rights_str_mv | © 2003, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc(Microbiology))--University of Pretoria, 2006. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24375 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:12.572Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24375 Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms Nel, Louis Hendrik upetd@up.ac.za Venter, S.N. (Stephanus Nicolaas) Mphahlele, M.T. (Matsie Theodora) Tuberculosis research Microbacterium tuberculosis Dna fingerpronting UCTD Dissertation (MSc(Microbiology))--University of Pretoria, 2006. DNA fingerprinting of Mycobacterium tuberculosis strain has been used in combination with conventional epidemiologic investigation, which has improved the understanding of tuberculosis transmission. Restriction Fragment Length Polymorphism (RFLP) based on IS6110 probe has become a standard method of fingerprinting of M tuberculosis. Since the technique is labour intensive and the discriminatory power of IS611 0 fingerprinting method for strains habouring only one to five copies is poor, other typing methods for typing M tuberculosis should be evaluated. In this regard, Amplified Fragment Length Polymorphism (AFLP) has the potential to overcome many of the RFLP problems. The first objective was to determine the suitability of the RFLP and AFLP techniques and to study the extent of transmission of tuberculosis in a referral hospital in South Africa. A total of 47 M tuberculosis isolates were differentiated using RFLP technique. The same samples were typed using the PCR- based AFLP technique and results were compared. The second objective was to determine the prevalence of isoniazid (INH) resistance and estimate the incidence of recent transmission of the disease in the Eastern-Cape (EC) and North-West province (NW) by using the best suited technique. RFLP grouped the 47 typed M. tuberculosis isolates into five families and four clusters. AFLP grouped the analyzed isolates (previously typed by RFLP) into two groups based on the banding patterns observed. As a result of the low degree of genotypic variation among the AFLP band pattern of M tuberculosis isolates, AFLP seemed less promising for individual strain differentiation of M tuberculosis. This technique can be used in future for differentiation of Mycobacterial species and The prevalence of INH resistance was found to be 6.7% in the EC and 8.4% in the NW province. The magnitude of recent transmission in the Eastern Cape studied by RFLP method, was found to be at 22% among the positive tuberculosis isolates identified. Transmission of TB in NW province was associated with reactivation rather than recent transmission due to lack of clustering of strains in that region. Microbiology and Plant Pathology unrestricted 2013-09-06T17:20:07Z 2005-05-19 2013-09-06T17:20:07Z 2004-04-24 2006-05-19 2005-05-06 Dissertation Mphahlele, MT 2003, Strain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24375 > H699/ag http://hdl.handle.net/2263/24375 http://upetd.up.ac.za/thesis/available/etd-05062005-140911/ © 2003, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Tuberculosis research Microbacterium tuberculosis Dna fingerpronting UCTD Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| title | Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| title_full | Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| title_fullStr | Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| title_full_unstemmed | Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| title_short | Stain differentiation of South African clinical isolates of Mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| title_sort | stain differentiation of south african clinical isolates of mycobacterium tuberculosis by restriction and amplified fragment length polymorphisms |
| topic | Tuberculosis research Microbacterium tuberculosis Dna fingerpronting UCTD |
| url | http://hdl.handle.net/2263/24375 http://upetd.up.ac.za/thesis/available/etd-05062005-140911/ |