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The protective role of oryzacystatin-1 under abiotic stress
Dissertation (MSc(Botany))--University of Pretoria, 2006.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613649396301824 |
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| access_status_str | Open Access |
| author2 | Kunert, Karl J. |
| author_browse | Kunert, Karl J. |
| author_facet | Kunert, Karl J. |
| collection | Thesis |
| dc_rights_str_mv | © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc(Botany))--University of Pretoria, 2006. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24479 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:30.213Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24479 The protective role of oryzacystatin-1 under abiotic stress Kunert, Karl J. upetd@up.ac.za Oberholster, A.M. Prins, Anneke Enzymes protection Crops effect of stress on Plants protection UCTD Dissertation (MSc(Botany))--University of Pretoria, 2006. One of the most important photosynthetic enzymes in a plant is ribulose-1,5¬bisphosphate carboxylase/oxygenase (Rubisco), which plays a key role in carbon fixation. Degradation of this enzyme leads to decreased carbon fixation and poor photosynthetic performance by the plant. It is therefore of interest to investigate possible ways of protecting this enzyme during stress conditions in order to generate plants that would perform better under extreme climates. In this study the effect of an expressed, exogenous rice cysteine proteinase inhibitor (OCI) in transformed tobacco plants on Rubisco stability/content under chilling and senescence was investigated. Results showed that there is no significant protective role for exogenous OCI on the degradation/content of Rubisco when tobacco plants were exposed to chilling. This result was found using native gel-based quantification procedures, as well as immuno-blotting, spot densitometric analysis, and a radioactive quantification assay as analysis techniques. The study, however, provided evidence for protection of Rubisco against degradation by expression of OCI under a more severe stress condition, such as senescence using native gel-based quantification procedures as detection techniques. Tobacco plants were also transformed with a newly designed vector allowing expressed OCI to be transported to the chloroplast. Failure to detect so far any OCI-¬expressing transformed plants and the idea that delay of senescence could prove beneficial to farmers by providing a more nutrient-dense crop with higher tolerance against stress-induced cell death are discussed. Plant Science unrestricted 2013-09-06T17:38:44Z 2005-05-19 2013-09-06T17:38:44Z 2003-09-05 2006-05-19 2005-05-09 Dissertation Prins, A 2003 The protective role of Oryzacystatin-1 under abiotic stress, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24479 > H233/ag http://hdl.handle.net/2263/24479 http://upetd.up.ac.za/thesis/available/etd-05092005-112847/ © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Enzymes protection Crops effect of stress on Plants protection UCTD The protective role of oryzacystatin-1 under abiotic stress |
| title | The protective role of oryzacystatin-1 under abiotic stress |
| title_full | The protective role of oryzacystatin-1 under abiotic stress |
| title_fullStr | The protective role of oryzacystatin-1 under abiotic stress |
| title_full_unstemmed | The protective role of oryzacystatin-1 under abiotic stress |
| title_short | The protective role of oryzacystatin-1 under abiotic stress |
| title_sort | protective role of oryzacystatin 1 under abiotic stress |
| topic | Enzymes protection Crops effect of stress on Plants protection UCTD |
| url | http://hdl.handle.net/2263/24479 http://upetd.up.ac.za/thesis/available/etd-05092005-112847/ |