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The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae
Dissertation (MSc)--University of Pretoria, 2012.
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University of Pretoria
2013
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| _version_ | 1867613718468100096 |
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| access_status_str | Open Access |
| author2 | Meyer, J.J.M. (Jacobus Johannes Marion) |
| author_browse | Meyer, J.J.M. (Jacobus Johannes Marion) |
| author_facet | Meyer, J.J.M. (Jacobus Johannes Marion) |
| collection | Thesis |
| dc_rights_str_mv | © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24657 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:36.114Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24657 The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae Meyer, J.J.M. (Jacobus Johannes Marion) fansie@live.co.za Hendriks, Christian Barend Stephanus Dichapetalum cymosum Dichapetalaceae Fluorinated compounds Tapura fischeri Virus-like particle (VLP) Endophytes Phytoferritin Transmission electron microscopy (TEM) UCTD Dissertation (MSc)--University of Pretoria, 2012. Dichapetalum cymosum (poison leaf) is a very common problem plant in southern Africa. Fluoroacetic acid, believed to be the poisonous entity in the plant, is produced by the plant, but the micro-organisms associated with this plant may also play a role in the production thereof. A previous study on Burolderia cepacia, an endophyte of D. cymosum showed active metabolism of fluoroacetate by this endophyte. The isolated endophytes from D. cymosum were studied to determine whether they synthesise any fluorinated compounds. It seemed from preliminary results that symbionts might play a role in the synthesis of the poisonous entities in D. cymosum, but further investigation is required. The detection of glandular lesions on the abaxial side of the leaf led to closer examination and the cross sections revealed unusually deformed epidermis cells with adjacent cells containing vacuoles filled with phenolic-like crystals. Transmission electron microscopy (TEM) of the spongy parenchyma cells directly above the glandular lesions indicated the presence of clusters of small, virus-like particles (VLPs) in the chloroplasts. Observations by TEM showed that these VLPs have analogous structures to phytoferritin. Tapura fischeri (leafberry tree) is a tree member of the same family, and it was found to also contain a fluorinated compound. Endophytes were also found in the plant and similar glandular lesions with analogous VLPs were observed at these sites. This might indicate that endophytes have a share in the biosynthesis of the fluorinated compounds found in Dichapetalaceae. Numerous factors ought to be considered in order to fully understand the chemical ecology of the intricate system regarding the endophytes and the possible toxicity of the family Dichapetalaceae. Plant Science unrestricted 2013-09-06T18:07:18Z 2013-05-22 2013-09-06T18:07:18Z 2013-04-12 2012 2013-05-14 Dissertation Hendriks, CBS 2012, The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24657 > E13/4/449/gm http://hdl.handle.net/2263/24657 http://upetd.up.ac.za/thesis/available/etd-05142013-123600/ © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Dichapetalum cymosum Dichapetalaceae Fluorinated compounds Tapura fischeri Virus-like particle (VLP) Endophytes Phytoferritin Transmission electron microscopy (TEM) UCTD The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae |
| title | The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae |
| title_full | The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae |
| title_fullStr | The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae |
| title_full_unstemmed | The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae |
| title_short | The role of endophytes in the metabolism of fluorinated compounds in the South African Dichapetalaceae |
| title_sort | role of endophytes in the metabolism of fluorinated compounds in the south african dichapetalaceae |
| topic | Dichapetalum cymosum Dichapetalaceae Fluorinated compounds Tapura fischeri Virus-like particle (VLP) Endophytes Phytoferritin Transmission electron microscopy (TEM) UCTD |
| url | http://hdl.handle.net/2263/24657 http://upetd.up.ac.za/thesis/available/etd-05142013-123600/ |