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Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco
Dissertation (MSc)--University of Pretoria, 2012.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613676778815488 |
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| access_status_str | Open Access |
| author2 | Kunert, Karl J. |
| author_browse | Kunert, Karl J. |
| author_facet | Kunert, Karl J. |
| collection | Thesis |
| dc_rights_str_mv | © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24658 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:56.368Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24658 Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco Kunert, Karl J. Vorster, Barend Juan tsholofelo.kibido@fabi.up.ac.za Kibido, Tsholofelo Reineth Glutathione reductase (gr) Transgenic tobacco plants UCTD Dissertation (MSc)--University of Pretoria, 2012. Protein degradation poses a significant challenge for the efficient production of recombinant proteins in plants, affecting the stability and yield of the recombinant protein. In this study the E. coli-derived enzyme glutathione reductase (GR) was transiently expressed in transgenic tobacco plants constitutively expressing the cysteine protease inhibitor OC-I and non-transgenic plants. A protein resembling the GR was detected in infiltrated leaves. Transiently expressing GR in transgenic N tabacum plants resulted in almost two fold significant increases in GR activity. Transgenic tobacco plants expressing the rice cysteine protease inhibitor OC-I had significantly lower cysteine protease activity when compared to non-transgenic tobacco plants. Lower cysteine protease activity in transgenic plants was directly related to higher GR activity and also higher GR amounts in transgenic plants. The study has demonstrated that OC-I is an effective companion protease inhibitor candidate with the potential to protect other high value proteins such as GR, from cysteine protease degradation. Plant Science unrestricted 2013-09-06T18:07:21Z 2013-05-24 2013-09-06T18:07:21Z 2013-04-12 2012 2013-05-14 Dissertation Kibido, TR 2012, Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24658 > E13/4/453/gm http://hdl.handle.net/2263/24658 http://upetd.up.ac.za/thesis/available/etd-05142013-135140/ © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Glutathione reductase (gr) Transgenic tobacco plants UCTD Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco |
| title | Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco |
| title_full | Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco |
| title_fullStr | Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco |
| title_full_unstemmed | Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco |
| title_short | Protection of recombinant glutathione reductase by Oryzacystatin-I in transgenic tobacco |
| title_sort | protection of recombinant glutathione reductase by oryzacystatin i in transgenic tobacco |
| topic | Glutathione reductase (gr) Transgenic tobacco plants UCTD |
| url | http://hdl.handle.net/2263/24658 http://upetd.up.ac.za/thesis/available/etd-05142013-135140/ |