Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens
Dissertation (MSc)--University of Pretoria, 2012.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2013
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613443357409280 |
|---|---|
| access_status_str | Open Access |
| author2 | Lall, Namrita |
| author_browse | Lall, Namrita |
| author_facet | Lall, Namrita |
| collection | Thesis |
| dc_rights_str_mv | © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24659 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:36:13.892Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24659 Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens Lall, Namrita Tshikalange, T.E. (Thilivhali Emmanuel) garland.more@up.ac.za More, Garland Kgosi Infections Artemisia afra UCTD Dissertation (MSc)--University of Pretoria, 2012. The phytochemical investigation of an ethanol extract of Artemisia afra, led to the isolation of six known compounds, Acacetin (1) 12α,4α-dihydroxybishopsolicepolide (2), Scopoletin (3) α-amyrin (4), Phytol (5) and a pentacyclic tri-terpenoid Betulinic acid (6). The isolated compounds were evaluated for their anti-microbial activity against Gram positive (Actinomyces naeslundii, Actinomyces israelii and Streptococcus mutans), Gram negative bacteria (Privotella intermedia, Porphyromonus gingivalis and Aggregatibacter actinomycetemcomitans previously known as Actinobacillus actinomycetemcomitans) and Candida albicans. The crude extract of A. afra inhibited the growth of all tested microbial species at concentration range of 1.6 mg/ml to 25.0 mg/ml. The compounds 1-6 also showed activity range at 1.0 mg/ml to 0.25 mg/ml. Three best compounds which showed good activity were selected for further studies. Cytotoxicity of the extract and compounds was determined using the XTT (Sodium 3’-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate) cell proliferation kit. The antioxidant activity of the extract and compounds was done using the DPPH scavenging method. The extract showed good antioxidant activity with an IC50 value of 22.2 μg/ml. Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC50 value of 1.24 μg/ml which was significant to that of vitamin C (1.22 μg/ml). Acacetin and Betulinic acid exhibited a decreased scavenging activity with the IC50 of 2.39 and 2.42 _g/ml, respectively. The extract and compounds showed moderate toxicity on McCoy fibroblast cell line and the extract influenced the release of cytokine against Hep2 cells. Scopoletin was relatively non-toxic with an IC50 value of 132.5 μg/ml. Acacetin and betulinic acid also showed a smooth trend of non-toxic effects at lower concentrations and toxic at higher concentrations with IC50 values of 35.44 and 30.96 μg/ml. The obtained results in this confirmed the use of A. afra in the treatment of microbial infections. Plant Science unrestricted 2013-09-06T18:07:24Z 2013-05-24 2013-09-06T18:07:24Z 2013-04-12 2012 2013-05-14 Dissertation More, GK 2012, Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24659 > E13/4/462/gm http://hdl.handle.net/2263/24659 http://upetd.up.ac.za/thesis/available/etd-05142013-174352/ © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Infections Artemisia afra UCTD Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens |
| title | Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens |
| title_full | Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens |
| title_fullStr | Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens |
| title_full_unstemmed | Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens |
| title_short | Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens |
| title_sort | antimicrobial constituents of artemisia afra jacq ex willd against periodontal pathogens |
| topic | Infections Artemisia afra UCTD |
| url | http://hdl.handle.net/2263/24659 http://upetd.up.ac.za/thesis/available/etd-05142013-174352/ |