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The application of a PCR based species identification method to African wildlife
Dissertation (MSc)--University of Pretoria, 2012.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613448354922496 |
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| access_status_str | Open Access |
| author2 | Harper, Cindy Kim |
| author_browse | Harper, Cindy Kim |
| author_facet | Harper, Cindy Kim |
| collection | Thesis |
| dc_rights_str_mv | © 2012, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/24803 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:36:18.633Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/24803 The application of a PCR based species identification method to African wildlife Harper, Cindy Kim Guthrie, Alan John leedarwent@live.com Darwent, Lee African wildlife species Domestic animals Species UCTD Dissertation (MSc)--University of Pretoria, 2012. Molecular based species identification is a useful tool in forensic investigations as well as routine work. The ability to cheaply and quickly determine the species of origin of a sample has become increasingly necessary as incidents of wildlife poaching, illegal meat trade and the trade of wildlife products has increased. The current methods of species identification tend to be expensive, time consuming and unreliable. The use of species-specific primers designed to bind to specific areas in the mitochondrial DNA, has been published. This application has been developed for a small number of domestic animals, however the application of these primers to African wildlife species has yet to be done. This method is relatively simple and is based on specific fragment size amplification using polymerase chain reaction and genotyping. A total of thirty seven different species were analyzed with this method and 30 of these species were found to have species-specific fragment sizes. A number of different sample types and conditions were tested including uncommon diagnostic samples such as rhinoceros horn, teeth and claws. In addition, the sensitivity of the method was investigated and determined to be very high, detecting species at a DNA concentration of just 0.1 pg/μl. This method was found to be a highly sensitive, efficient and a fast way to determine species in a number of different sample types and would therefore be of great value in the wildlife trade as these samples can often be of a lower quality or only available in small amounts. The use of this method in forensic science must be done with care due to the problem of cross species amplification. In addition not all of the African Wildlife mammals were available to test, limiting the detection power and specificity of the test. Copyright Production Animal Studies unrestricted 2013-09-06T18:28:01Z 2012-05-21 2013-09-06T18:28:01Z 2012-04-13 2012-05-21 2012-05-18 Dissertation Darwent, L 2012, The application of a PCR based species identification method to African wildlife, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/24803 > E12/4/290/gm http://hdl.handle.net/2263/24803 http://upetd.up.ac.za/thesis/available/etd-05182012-153455/ © 2012, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | African wildlife species Domestic animals Species UCTD The application of a PCR based species identification method to African wildlife |
| title | The application of a PCR based species identification method to African wildlife |
| title_full | The application of a PCR based species identification method to African wildlife |
| title_fullStr | The application of a PCR based species identification method to African wildlife |
| title_full_unstemmed | The application of a PCR based species identification method to African wildlife |
| title_short | The application of a PCR based species identification method to African wildlife |
| title_sort | application of a pcr based species identification method to african wildlife |
| topic | African wildlife species Domestic animals Species UCTD |
| url | http://hdl.handle.net/2263/24803 http://upetd.up.ac.za/thesis/available/etd-05182012-153455/ |