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Characterization of two plant rhabdoviruses not previously reported in South Africa
Dissertation (MSc)--University of Pretoria, 2011.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613622826434560 |
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| access_status_str | Open Access |
| author2 | Nel, Louis Hendrik |
| author_browse | Nel, Louis Hendrik |
| author_facet | Nel, Louis Hendrik |
| collection | Thesis |
| dc_rights_str_mv | © 2008 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2011. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/25340 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:04.809Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/25340 Characterization of two plant rhabdoviruses not previously reported in South Africa Nel, Louis Hendrik Pietersen, Gerhard s23089416@tuks.co.za Lamprecht, Renate Luise Bermuda grass Rhabdoviruses UCTD Dissertation (MSc)--University of Pretoria, 2011. Two previously uncharacterized plant rhabdoviruses, infecting Bermuda grass (Cynodon dactylon (L.) Pers) and soybean (Glycine max) respectively, have been found in South Africa. To determine the morphology and virion size of these viruses, embedded ultra-thin sections of infected plant samples were observed under a transmission electron microscope. The virion distribution within the cell, the bulletshaped morphology and the virion sizes indicated that both these viruses might belong to the Rhabdoviridae family. Degenerate polymerase chain reaction (PCR) primers were designed by alignment of the polymerase gene sequences of several plant rhabdoviruses in order to identify conserved regions. Standard PCR and sequencing protocols were used to determine a partial polymerase gene sequence of the viruses that was then compared to the most closely related sequences available on Genbank. The analysis indicated that the Cynodon rhabdovirus was most closely related to known nucleorhabdoviruses; and the rhabdovirus-infecting soybean (Soybean blotching mosaic virus proposed name) was closely related to other known cytorhabdoviruses. These results indicate that both the viruses are new members to the Nucleo- and Cytorhabdovirus genera, respectively. Microbiology and Plant Pathology unrestricted 2013-09-06T20:49:44Z 2009-06-18 2013-09-06T20:49:44Z 2009-04-15 2011-06-15 2009-06-08 Dissertation Lamprecht, RL 2008, Characterization of two plant rhabdoviruses not previously reported in South Africa, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/25340 > E1393/ag http://hdl.handle.net/2263/25340 http://upetd.up.ac.za/thesis/available/etd-06082009-154402/ © 2008 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Bermuda grass Rhabdoviruses UCTD Characterization of two plant rhabdoviruses not previously reported in South Africa |
| title | Characterization of two plant rhabdoviruses not previously reported in South Africa |
| title_full | Characterization of two plant rhabdoviruses not previously reported in South Africa |
| title_fullStr | Characterization of two plant rhabdoviruses not previously reported in South Africa |
| title_full_unstemmed | Characterization of two plant rhabdoviruses not previously reported in South Africa |
| title_short | Characterization of two plant rhabdoviruses not previously reported in South Africa |
| title_sort | characterization of two plant rhabdoviruses not previously reported in south africa |
| topic | Bermuda grass Rhabdoviruses UCTD |
| url | http://hdl.handle.net/2263/25340 http://upetd.up.ac.za/thesis/available/etd-06082009-154402/ |