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Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor
Dissertation (MEng)--University of Pretoria, 2012.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613515880071168 |
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| access_status_str | Open Access |
| author2 | Nicol, Willie |
| author_browse | Nicol, Willie |
| author_facet | Nicol, Willie |
| collection | Thesis |
| dc_rights_str_mv | © 2012, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MEng)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/25834 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:22.997Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/25834 Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor Nicol, Willie mundu.jm@gmail.com Mwakio, Joseph Mundu Continuous succinic acid production actinobacillus succinogenes Biofilm UCTD Dissertation (MEng)--University of Pretoria, 2012. Succinic Acid (SA) was continuously produced using glucose and a Mg2CO3(OH)2 slurry as feed. Glucose feed concentrations of 20 and 40 g ℓ-1 were employed with corresponding Mg2CO3(OH)2 slurry concentrations of 60 and 120 g ℓ-1. The reactor pH was passively maintained between 6,4 and 6,8 by the buffer properties of the slurry in conjunction with the pH adjusted glucose feed. The suspended cell (SC) reactor was operated at 37°C with dilution rates varying between 0,04 h-1and 0,6 h-1. Groperl® particles were used as inert supports in the biofilm reactor; dilution rates of 0,11 h-1 to 1 h-1 were investigated. Two SC fermentations were conducted for the 20 g ℓ-1 glucose feed concentration and one for the 40 g ℓ-1. All SC fermentation runs were operated in excess of 12 days, while the biofilm run lasted 6,5 days. Fermentations were terminated only after contamination by lactic acid bacteria was observed. SC fermentations with the glucose feed concentration of 20 g ℓ-1 achieved a maximum SA productivity of 5,2 g ℓ-1h-1 at 0,6 h-1 with a corresponding SA yield of 0,65 g g-1. SC fermentations with the glucose feed concentration of 40 g ℓ-1 achieved a maximum SA productivity of 3,76 g ℓ-1h-1 at 0,4 h-1 with a SA yield of 0,82 g g-1. The results were comparable to the other continuous studies with Actinobacillus succinogenes, despite the fact that either biofilms or membranes were employed in these studies. The preliminary biofilm study demonstrated the capability of A. succinogenes to produce SA in high productivities and yields. SA productivities and yields for the dilution rates of 0,33 h-1 and 1,0 h-1, were 5,72 g ℓ-1h-1 (0,95 g g-1) and 12 g ℓ-1h-1 (1,0 g g-1), respectively. The biofilm reactor at 0,33 h-1 achieved twice the SA productivity of the SC reactor at 0,3 h-1 with a 42 % increase in SA yield. Copyright Chemical Engineering unrestricted 2013-09-07T00:51:17Z 2012-06-26 2013-09-07T00:51:17Z 2012-04-17 2012-06-26 2012-06-25 Dissertation Mwakio, JM 2012, Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor, MEng dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/25834 > E12/4/419/gm http://hdl.handle.net/2263/25834 http://upetd.up.ac.za/thesis/available/etd-06252012-104038/ © 2012, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Continuous succinic acid production actinobacillus succinogenes Biofilm UCTD Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor |
| title | Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor |
| title_full | Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor |
| title_fullStr | Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor |
| title_full_unstemmed | Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor |
| title_short | Continuous succinic acid production by Actinobacillus Succinogenes : suspended cell and biofilm studies in an anaerobic slurry reactor |
| title_sort | continuous succinic acid production by actinobacillus succinogenes suspended cell and biofilm studies in an anaerobic slurry reactor |
| topic | Continuous succinic acid production actinobacillus succinogenes Biofilm UCTD |
| url | http://hdl.handle.net/2263/25834 http://upetd.up.ac.za/thesis/available/etd-06252012-104038/ |