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Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.)
Dissertation (MSc)--University of Pretoria, 2011.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613535814549504 |
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| access_status_str | Open Access |
| author2 | Steenkamp, Vanessa |
| author_browse | Steenkamp, Vanessa |
| author_facet | Steenkamp, Vanessa |
| collection | Thesis |
| dc_rights_str_mv | © 2010, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2011. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26131 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:41.969Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26131 Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) Steenkamp, Vanessa capallant@gmail.com Cromarty, Allan Duncan Pallant, Christopher Alexander Methicillin-resistant staphylococcus aureus Indole alkaloids Mrsa Antibacterial natural products Tabernaemontana elegans Tuberculosis (TB) UCTD Dissertation (MSc)--University of Pretoria, 2011. Bacterial infections remain a significant threat to human health. Due to the emergence of widespread antibiotic resistance, development of novel antibiotics is required in order to ensure that effective treatment remains available. The aim of this study was to isolate and identify the fraction responsible for the antimicrobial activity in Tabernaemontana elegans (Stapf.) root extracts. The active fraction was characterised by thin layer chromatography (TLC) and gas chromatography – mass spectrometry (GC-MS). Antibacterial activity was determined using the broth micro-dilution assay and antimycobacterial activity using the BACTEC radiometric assay. Cytotoxicity of the crude extract and fractions was assessed against primary cell cultures; lymphocytes and fibroblasts; as well as a hepatocarcinoma (HepG2) and macrophage (THP-1) cell line using the Neutral Red uptake and MTT assays. The crude root extracts were found to contain a high concentration of alkaloids (1.2% w/w). GC-MS analysis identified the indole alkaloids, voacangine and dregamine, as major components. Antibacterial activity was limited to the Gram-positive bacteria and Mycobacterium species, with MIC values in the range of 64 – 256 ìg/ml. When combined with antibiotics, additive antibacterial effects were observed. Marked cytotoxicity to all cell lines tested was evident in the MTT and Neutral Red uptake assays, with IC50 values ranging between 1.11 – 9.81 ìg/ml. This study confirms the antibacterial activity of T. elegans and supports its potential for being investigated further for the development of a novel antibacterial compound. Pharmacology unrestricted 2013-09-07T02:41:23Z 2011-07-14 2013-09-07T02:41:23Z 2011-04-08 2011-07-14 2011-07-08 Dissertation Pallant, CA 2010, Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.), MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26131 > E11/304/gm http://hdl.handle.net/2263/26131 http://upetd.up.ac.za/thesis/available/etd-07082011-165915/ © 2010, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Methicillin-resistant staphylococcus aureus Indole alkaloids Mrsa Antibacterial natural products Tabernaemontana elegans Tuberculosis (TB) UCTD Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) |
| title | Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) |
| title_full | Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) |
| title_fullStr | Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) |
| title_full_unstemmed | Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) |
| title_short | Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.) |
| title_sort | bioactivity of the alkaloidal fraction of tabermaemintana elegans stapf |
| topic | Methicillin-resistant staphylococcus aureus Indole alkaloids Mrsa Antibacterial natural products Tabernaemontana elegans Tuberculosis (TB) UCTD |
| url | http://hdl.handle.net/2263/26131 http://upetd.up.ac.za/thesis/available/etd-07082011-165915/ |