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The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR
Dissertation (MSc)--University of Pretoria, 2012.
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University of Pretoria
2013
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| _version_ | 1867613606185533440 |
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| access_status_str | Open Access |
| author2 | Collins, Nicola E. |
| author_browse | Collins, Nicola E. |
| author_facet | Collins, Nicola E. |
| collection | Thesis |
| dc_rights_str_mv | © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
| description | Dissertation (MSc)--University of Pretoria, 2012. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26197 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:38:49.043Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26197 The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR Collins, Nicola E. Toye, Philip G. Oosthuizen, Marinda C. Sibeko, K.P. (Kgomotso Penelope) enjiiri@gmail.com Njiiri, Evalyne Nyawira Haemoparasites Ehrlichia ruminantium UCTD Dissertation (MSc)--University of Pretoria, 2012. Ehrlichia ruminantium is a tick-borne pathogen transmitted by ticks in the genus Amblyomma. This bacterial pathogen causes heartwater, a fatal disease affecting domestic and wild ruminants in sub-Saharan Africa. The prevalence of heartwater in western Kenya is not well documented. In this study, reverse line blot (RLB) hybridization and quantitative real-time PCR (qPCR) assays were used to detect E. ruminantium DNA in 545 blood samples collected from calves from twenty sublocations distributed across five agro-ecological zones of western Kenya. Ehrlichia ruminantium DNA was detected in 1.10% and 0.92% of the samples using RLB and qPCR, respectively. There were discrepancies in the detection of E. ruminantium by the RLB and the qPCR. Five samples were positive with the qPCR while six were positive with the RLB, but only three of the samples were positive by both tests. The occurrence of E. ruminantium in western Kenya appears to be low, but this might be attributed to the inability of the tests used to detect E. ruminantium carriers. The most prevalent haemoparasites detected by the RLB in the Ehrlichia/Anaplasma group were Anaplasma (formerly Ehrlichia) sp. Omatjenne and Anaplasma bovis at 37.98% each, while Theileria mutans (66.61%) was the most prevalent in the Theileria/Babesia group. In addition, a nested p104 PCR was used to detect Theileria parva in a subset of 86 of the samples; T. parva was detected in 32.56% (28/86) of these samples. The RLB detected T. parva in 27.91% (24/86) of the same sample subset, but only 17 were positive by both tests. The molecular tests used in this study suggest that, of the pathogenic haemoparasites known to cause disease in Kenya, T. parva occurs the most commonly in western Kenya, while E. ruminantium, A. marginale, B. bigemina and B. bovis are less frequently detected. Veterinary Tropical Diseases unrestricted 2013-09-07T03:43:47Z 2013-07-12 2013-09-07T03:43:47Z 2013-04-12 2013-07-12 2013-07-10 Dissertation Njiiri, EN 2012, The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26197 > F13/4/674/gm http://hdl.handle.net/2263/26197 http://upetd.up.ac.za/thesis/available/etd-07102013-172020/ © 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria application/pdf University of Pretoria |
| spellingShingle | Haemoparasites Ehrlichia ruminantium UCTD The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR |
| title | The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR |
| title_full | The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR |
| title_fullStr | The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR |
| title_full_unstemmed | The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR |
| title_short | The occurrence of Ehrlichia ruminantium and other haemoparasites in calves in western Kenya determined by reverse line blot hybridization assay, real-time PCR and nested PCR |
| title_sort | occurrence of ehrlichia ruminantium and other haemoparasites in calves in western kenya determined by reverse line blot hybridization assay real time pcr and nested pcr |
| topic | Haemoparasites Ehrlichia ruminantium UCTD |
| url | http://hdl.handle.net/2263/26197 http://upetd.up.ac.za/thesis/available/etd-07102013-172020/ |