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Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea
Thesis (PhD (Microbiology))--University of Pretoria, 2009.
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University of Pretoria
2013
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| _version_ | 1867613669566709760 |
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| access_status_str | Open Access |
| author2 | Coutinho, Teresa A. |
| author_browse | Coutinho, Teresa A. |
| author_facet | Coutinho, Teresa A. |
| collection | Thesis |
| dc_rights_str_mv | © 2007, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Microbiology))--University of Pretoria, 2009. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26297 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:49.604Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26297 Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea Coutinho, Teresa A. Venter, S.N. (Stephanus Nicolaas) upetd@up.ac.za Goszczynska, Teresa Seed-borne in onions Pantoea ananatis UCTD Thesis (PhD (Microbiology))--University of Pretoria, 2009. Center rot of onion, caused by Pantoea ananatis, was first described in the USA, in 1997. P. ananatis is seed-borne in onions and it was suggested that it was introduced into the USA on infected seed lots from South Africa. Center rot has not been observed in South Africa and it was essential to determine if P. ananatis is present in local onion seed. Colonies resembling those of P. ananatis were isolated from four South African seed lots on PA 20, a new semi-selective medium. Pathogenicity tests demonstrated that the South African and America strains induced the same symptoms on onion. Phenotypic and genotypic analyses identified the strains from seed as P. ananatis. In 2004/2005, an unreported disease of maize, brown stalk rot, was observed on commercial fields in South Africa. The representative strains induced disease symptoms similar to those observed in the field. The phenotypic and genotyping tests showed that the strains belonged to the genus Pantoea and separated them into two groups. The first group was identified as P. ananatis. The F-AFLP genomic fingerprints generated by the second group of strains, were distinctly different from those generated by known Pantoea species. To resolve the taxonomic position of Pantoea isolated from onion and maize, sixty-seven strains were subjected to a polyphasic study. The methods used included phenotypic characterisation, genomic fingerprinting, 16S rRNA gene sequence analysis and DNA-DNA hybridisation. The results revealed that the strains belong to three different species within the genus Pantoea: P. ananatis, P. vagens and a novel species, Pantoea allii sp. nov. Microbiology and Plant Pathology unrestricted 2013-09-07T04:26:00Z 2008-08-12 2013-09-07T04:26:00Z 2007-09-06 2009-08-12 2008-07-15 Thesis Goszczynska, T 2007, Emerging diseases of maize and onion caused by bacteria belonging to the Genus Pantoea, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26297 > D378/ag http://hdl.handle.net/2263/26297 http://upetd.up.ac.za/thesis/available/etd-07152008-083553/ © 2007, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf application/pdf application/pdf application/pdf application/pdf University of Pretoria |
| spellingShingle | Seed-borne in onions Pantoea ananatis UCTD Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea |
| title | Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea |
| title_full | Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea |
| title_fullStr | Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea |
| title_full_unstemmed | Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea |
| title_short | Emerging diseases of maize and onion caused by bacteria belonging to the genus Pantoea |
| title_sort | emerging diseases of maize and onion caused by bacteria belonging to the genus pantoea |
| topic | Seed-borne in onions Pantoea ananatis UCTD |
| url | http://hdl.handle.net/2263/26297 http://upetd.up.ac.za/thesis/available/etd-07152008-083553/ |