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Towards the diagnosis of two intracellular pathogens of grapevine in South Africa
Dissertation (MSc (Microbiology))--University of Pretoria, 2010.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613632068583424 |
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| access_status_str | Open Access |
| author2 | Pietersen, Gerhard |
| author_browse | Pietersen, Gerhard |
| author_facet | Pietersen, Gerhard |
| collection | Thesis |
| dc_rights_str_mv | © 2006 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2010. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26306 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:13.632Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26306 Towards the diagnosis of two intracellular pathogens of grapevine in South Africa Pietersen, Gerhard Nel, Louis Hendrik okoch@tuks.co.za Koch, Orienka Intracellular pathogens of grapevine South africa UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2010. A survey was conducted, from 2001 to 2004, of viruses spreading within certified grapevine material in South Africa. As far as possible, viruses were identified and detection methods established. However, unknown spherical virus-like particles were observed in samples that also contained Grapevine Leafroll Associated Virus-Type 3. The unknown spherical particles were thought to most likely be Grapevine Fleck Virus, which was previously found in South Africa. A PCR method to be used locally for the routine detection of Grapevine Fleck Virus was established and first used to determine whether any of the greenhouse and field samples with the unknown spherical viruses were infected with Grapevine Fleck Virus. During the 2001 to 2004 survey, plants with leafroll and reddening symptoms unlike classical grapevine leafroll disease were also observed. No grapevine leafroll-associated viruses could be detected in these, but the symptoms observed resembled symptoms induced by phytoplasmas in Europe. A PCR method for the routine universal detection of phytoplasmas was established and this method was used to determine if phytoplasmas were associated with the symptomatic plants found. Sequence information from PCR amplicons suggest the presence of Candidatus phytoplasma solani, found for the first time in South Africa. This important finding however requires conformation by a second laboratory. Microbiology and Plant Pathology unrestricted 2013-09-07T04:31:16Z 2008-08-12 2013-09-07T04:31:16Z 2006-09-07 2010-08-12 2008-07-15 Dissertation Koch, O 2006, Towards the diagnosis of two intracellular pathogens of grapevine in South Africa, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26306 > E415/ag http://hdl.handle.net/2263/26306 http://upetd.up.ac.za/thesis/available/etd-07152008-144250/ © 2006 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Intracellular pathogens of grapevine South africa UCTD Towards the diagnosis of two intracellular pathogens of grapevine in South Africa |
| title | Towards the diagnosis of two intracellular pathogens of grapevine in South Africa |
| title_full | Towards the diagnosis of two intracellular pathogens of grapevine in South Africa |
| title_fullStr | Towards the diagnosis of two intracellular pathogens of grapevine in South Africa |
| title_full_unstemmed | Towards the diagnosis of two intracellular pathogens of grapevine in South Africa |
| title_short | Towards the diagnosis of two intracellular pathogens of grapevine in South Africa |
| title_sort | towards the diagnosis of two intracellular pathogens of grapevine in south africa |
| topic | Intracellular pathogens of grapevine South africa UCTD |
| url | http://hdl.handle.net/2263/26306 http://upetd.up.ac.za/thesis/available/etd-07152008-144250/ |