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Clinical relevance of Salmonella enterica isolated from water and food in Eritrea
Dissertation (MSc (Microbiology))--University of Pretoria, 2007.
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University of Pretoria
2013
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| _version_ | 1867613696081002496 |
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| access_status_str | Open Access |
| author2 | Venter, S.N. (Stephanus Nicolaas) |
| author_browse | Venter, S.N. (Stephanus Nicolaas) |
| author_facet | Venter, S.N. (Stephanus Nicolaas) |
| collection | Thesis |
| dc_rights_str_mv | © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2007. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26422 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:14.906Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26422 Clinical relevance of Salmonella enterica isolated from water and food in Eritrea Venter, S.N. (Stephanus Nicolaas) upetd@ais.up.ac.za Said, H.M. (Halima Mohammed) No key words available UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2007. A total of 94 Salmonella isolates were collected from three catchments areas in Eritrea. These isolates were recovered from clinical and environmental sources. Biochemical tests using gelatin hydrolysis and tartrate utilization test were employed to differentiate between Salmonellasubspecies. All Salmonellaisolates were identified as Salmonellasubspecies I and were then subjected to molecular characterization. Polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP) were employed to identify and establish possible relationships between the clinical isolates and environmental sources. Two sets of oligonucleotide primers specific for genes from S. Typhimurium and S. Enteritidis were used for the PCR reaction. Of the 94 Salmonellaisolates characterized only 6 were S. Typhimurium strains. To type the Salmonellaisolates AFLP was used. Clustering the AFLP patterns using the un-weighed pair-group method using arithmetic means (UPGMA) revealed 15 clusters. Of the 94 Salmonellaisolates collected, 48 (51%) strains were serologically identified. These serotypes include, 21 SalmonellaEmek (43.7%), 19 SalmonellaHeidelberg (39.5%), 7 of the 13, 22, 23; z undetermined serotype (14.5%), and 2 SalmonellaTyphimurium strains (4.1%). The AFLP data in the present study indicated a possible relationship between the clinical isolates and those obtained from environmental sources. Microbiology and Plant Pathology unrestricted 2013-09-07T05:21:02Z 2006-02-01 2013-09-07T05:21:02Z 2005-01-21 2007-02-01 2006-02-01 Dissertation Said, H 2005, Clinical relevance of Salmonella enterica isolated from water and food in Eritrea, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26422 > http://hdl.handle.net/2263/26422 http://upetd.up.ac.za/thesis/available/etd-02012006-112757/ © 2005, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | No key words available UCTD Clinical relevance of Salmonella enterica isolated from water and food in Eritrea |
| title | Clinical relevance of Salmonella enterica isolated from water and food in Eritrea |
| title_full | Clinical relevance of Salmonella enterica isolated from water and food in Eritrea |
| title_fullStr | Clinical relevance of Salmonella enterica isolated from water and food in Eritrea |
| title_full_unstemmed | Clinical relevance of Salmonella enterica isolated from water and food in Eritrea |
| title_short | Clinical relevance of Salmonella enterica isolated from water and food in Eritrea |
| title_sort | clinical relevance of salmonella enterica isolated from water and food in eritrea |
| topic | No key words available UCTD |
| url | http://hdl.handle.net/2263/26422 http://upetd.up.ac.za/thesis/available/etd-02012006-112757/ |