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Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro
Dissertation (MChD)--University of Pretoria, 2010.
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University of Pretoria
2013
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| _version_ | 1867613506319155201 |
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| access_status_str | Open Access |
| author2 | Botha, Stephanus Johannes |
| author_browse | Botha, Stephanus Johannes |
| author_facet | Botha, Stephanus Johannes |
| collection | Thesis |
| dc_rights_str_mv | © 2006 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MChD)--University of Pretoria, 2010. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26715 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:13.890Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26715 Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro Botha, Stephanus Johannes upetd@up.ac.za De Wet, Tanya Minocline and doxycycline concentrations UCTD Dissertation (MChD)--University of Pretoria, 2010. Minocycline (MC) and Doxycycline (DC) are used worldwide as locally applied adjuncts in the treatment of periodontal diseases. As a group the tetracyclines are well known for their advantageous properties. There is however possible cytotoxicity towards cells in the area of application. This study determined the minimum cytocidal concentration of MC and DC on the growth and proliferation of Human Periodontal Ligament Fibroblasts (HPLF) in vitro. This was facilitated by growing cells (PDL1 and PDL2) in the presence of MC and DC in media in 96 tissue wells starting at a concentration of 1400 µg.ml-1 (100%). Serial dilutions of the MC and DC at 10% increments were investigated in order to detect significant HPLF cell growth inhibition. The significant LD50 was further determined at one percent increments in order to arrive at a specific percentage value. The results were read as LD50 values from growth concentration curves. The LD50 of MC on PDL1 and PDL2 after one hour exposure was 686 µg.ml-1 and 896 µg.ml-1 respectively while for DC it was 252 µg.ml-1 and 546 µg.ml-1. The LD50 of MC on PDL1 and PDL2 after 24 hour exposure was 196 µg.ml-1 and 266 µg.ml-1 respectively while for DC it was 252 µg.ml-1 for both. The LD50 of MC on PDL1 and PDL2 after 48 hour exposure was 252 µg.ml-1 and 182 µg.ml-1 respectively while for DC it was 154 µg.ml-1 and 168 µg.ml-1. Based upon the LD50 values this study found that DC is more cytotoxic than MC and linked to this, the two cell lines reacted slightly differently. The concentrations MC and DC tested in this study did however not influence growth of HPLF significantly. Oral Pathology and Oral Biology unrestricted 2013-09-07T07:21:45Z 2010-07-27 2013-09-07T07:21:45Z 2006-11-01 2010-07-27 2010-07-27 Dissertation De Wet, T 2006, Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro, MChD dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26715 > H917/ag http://hdl.handle.net/2263/26715 http://upetd.up.ac.za/thesis/available/etd-07272010-110425/ © 2006 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Minocline and doxycycline concentrations UCTD Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| title | Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| title_full | Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| title_fullStr | Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| title_full_unstemmed | Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| title_short | Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| title_sort | minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro |
| topic | Minocline and doxycycline concentrations UCTD |
| url | http://hdl.handle.net/2263/26715 http://upetd.up.ac.za/thesis/available/etd-07272010-110425/ |