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Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products
Thesis (PhD)--University of Pretoria, 2007.
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University of Pretoria
2013
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| _version_ | 1867613519766093824 |
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| access_status_str | Open Access |
| author2 | Allsopp, Basil A. |
| author_browse | Allsopp, Basil A. |
| author_facet | Allsopp, Basil A. |
| collection | Thesis |
| dc_rights_str_mv | © 2007 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2007. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26758 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:26.754Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26758 Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products Allsopp, Basil A. Collins, Nicola E. pretoriusac@arc.agric.za Pretorius, Alri Ehrlichia ruminantium gene products UCTD Thesis (PhD)--University of Pretoria, 2007. In the search for a better vaccine against Ehrlichia ruminantium infection in ruminants, four E. ruminantium open reading frames (ORFs) derived from the Welgevonden isolate were tested using either DNA vaccination or DNA primemodified viral or DNA prime-recombinant protein boost strategies. Both the DNA vaccination and the DNA prime.recombinant protein boost strategy provided complete protection against E. ruminantium Welgevonden needle challenge, while the DNA prime.modified viral boost strategy only provided 90 % protection. The DNA prime.recombinant protein boost strategy also coincided with elevated cellular immunology as was evident from increased IFN-ã production. Furthermore, we could show that the 1H12 DNA vaccine could induce protection against heterologous needle challenge when animals were immunised with the Welgevonden-derived 1H12 ORFs and challenged with selected E. ruminantium stocks. Unfortunately the DNA only and the DNA prime.recombinant protein boost strategy were not protective in the field. Therefore, our results suggest that there is a vast difference between needle challenge and natural tick infestation and that E. ruminantium organisms transmitted by ticks have the ability to evade the protective immunity induced by immunization with the four 1H12 ORFs. Veterinary Tropical Diseases unrestricted 2013-09-07T07:50:53Z 2008-08-11 2013-09-07T07:50:53Z 2007-11-23 2008-08-11 2008-07-28 Thesis Pretorius, A 2007, Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products , PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26758 > D369/ag http://hdl.handle.net/2263/26758 http://upetd.up.ac.za/thesis/available/etd-07282008-144043/ © 2007 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Ehrlichia ruminantium gene products UCTD Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products |
| title | Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products |
| title_full | Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products |
| title_fullStr | Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products |
| title_full_unstemmed | Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products |
| title_short | Aspects of the immune response in ruminants to four protective Ehrlichia ruminantium gene products |
| title_sort | aspects of the immune response in ruminants to four protective ehrlichia ruminantium gene products |
| topic | Ehrlichia ruminantium gene products UCTD |
| url | http://hdl.handle.net/2263/26758 http://upetd.up.ac.za/thesis/available/etd-07282008-144043/ |