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Properties of anti-mycolic acid antibodies in human tuberculosis patients
Dissertation (MSc (Biochemistry))--University of Pretoria, 2007.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613455032254464 |
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| access_status_str | Open Access |
| author2 | Verschoor, Jan Adrianus |
| author_browse | Verschoor, Jan Adrianus |
| author_facet | Verschoor, Jan Adrianus |
| collection | Thesis |
| dc_rights_str_mv | © 2004, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Biochemistry))--University of Pretoria, 2007. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/26934 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:36:24.683Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/26934 Properties of anti-mycolic acid antibodies in human tuberculosis patients Verschoor, Jan Adrianus upetd@ais.up.ac.za Coetzer, T.H.T. Vermaak, Yvonne No key words availble UCTD Dissertation (MSc (Biochemistry))--University of Pretoria, 2007. Tuberculosis has re-emerged as a global health threat today. Current tuberculosis diagnosis is too slow in general and unsensitive in HIV burdened populations. Exposure to mycobacterial antigens in a country with a high prevalence of tuberculosis leads to false positive test results. Serodiagnosis would have been ideal, but was hitherto not successful. Mycolic acid (MA) is the major lipid cell wall constituent of Mycobacterium tuberculosis, the etiological agent of this disease. In this study an antibody response to the MA molecules are investigated as a possible surrogate marker for tuberculosis. In previous studies, IgG antibodies to MA in TB infected, HIV seronegative patients were detected in human sera, with promising results. In this study the ELISA results detecting anti-IgG antibodies to MA in TB and HIV co-infected patients showed a low sensitivity and specificity. The study, however, showed that antibodies to MA are prevalent in HIV seropositive patients. The presentation of MA on the CD1 molecule to T cells might explain why anti-MA antibodies are detected in HIV seropositive patients. The properties of anti-mycolic acid antibodies were investigated to explain the low sensitivities and specificities of the ELISA test. An ELISA was done comparing signals to MA and cholesterol as coating antigen. A degree of cross-reactivity of anti-MA antibodies to cholesterol was obtained. In using the IAsys biosensor it was shown that anti-MA antibodies were inhibited with MA and cholesterol as antigens in liposomes with cholesterol as the weaker antigen. An antibody response to MA might prove to be a good surrogate marker for tuberculosis when measured in an IAsys biosensor based serodiagnostic test, where the serodiagnosis does not depend on the detection of high affinity anti-MA antibodies only. Biochemistry unrestricted 2013-09-07T09:14:36Z 2006-02-06 2013-09-07T09:14:36Z 2004-11-19 2007-02-06 2006-02-06 Dissertation Vermaak, Y 2004, Properties of anti-mycolic acid antibodies in human tuberculosis patients, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/26934 > http://hdl.handle.net/2263/26934 http://upetd.up.ac.za/thesis/available/etd-02062006-141927/ © 2004, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | No key words availble UCTD Properties of anti-mycolic acid antibodies in human tuberculosis patients |
| title | Properties of anti-mycolic acid antibodies in human tuberculosis patients |
| title_full | Properties of anti-mycolic acid antibodies in human tuberculosis patients |
| title_fullStr | Properties of anti-mycolic acid antibodies in human tuberculosis patients |
| title_full_unstemmed | Properties of anti-mycolic acid antibodies in human tuberculosis patients |
| title_short | Properties of anti-mycolic acid antibodies in human tuberculosis patients |
| title_sort | properties of anti mycolic acid antibodies in human tuberculosis patients |
| topic | No key words availble UCTD |
| url | http://hdl.handle.net/2263/26934 http://upetd.up.ac.za/thesis/available/etd-02062006-141927/ |