Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa
Dissertation (MSc)--University of Pretoria, 2009.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2013
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613682306908160 |
|---|---|
| access_status_str | Open Access |
| author2 | Picard, J.A. |
| author_browse | Picard, J.A. |
| author_facet | Picard, J.A. |
| collection | Thesis |
| dc_rights_str_mv | © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2009. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/27117 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:40:01.664Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/27117 Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa Picard, J.A. annelizej@elsenburg.com Jonker, Annelize Poultry Broth microdilution Minimum inhibitory concentration Antimicrobial susceptibility Campylobacter coli Campylobacter jejuni Thermophilic Mic Pigs Gauteng Western Cape UCTD Dissertation (MSc)--University of Pretoria, 2009. The thermophilic Campylobacters, Campylobacter jejuni and Campylobacter coli are found as commensals in the intestinal tract of healthy mammals and birds. Campylobacter jejuni is one of the leading causes of sporadic food-borne bacterial disease in humans which is predominantly contracted from poultry products. Although the vast majority of these infections are mild, life-threatening complications should be treated with antimicrobials. Patients are usually treated with either macrolides of fluoroquinolones. However, globally there is an increased trend in the development of resistance to these antibiotics. This trend has also been observed in infection of poultry and pigs. The aim of this investigation was to determine antimicrobial sensitivity of thermophilic Campylobacters isolated from pigs and poultry by broth microdilution minimum inhibitory concentration testing. A total of 482 samples of the small intestinal content from poultry and pigs from the Western Cape and Gauteng Provinces were collected and analysed. Thirty-eight Campylobacter isolates were obtained. Statistical analyses included percentage resistance, minimum inhibitory concentrations (MIC50 and MIC90) as well as the distribution percentages of the MICs. The non-parametric Mann-Whitney U test was used to establish any significant differences at an interspecies, interhost and interprovincial level. Analyses of the data obtained revealed indications of decreasing susceptibility to several antibiotic groups including the tetracyclines, macrolides, erythromycin and tylosin, as well as the lincoasamides, and fluoroquinolones. It was found that isolates from the Western Cape were more likely to be resistant to the fluoroquinolones (p = 0.0392), macrolides (p = 0.0262), and lincoasmides (p = 0.0001) and, as well as to a certain extent the pleuromutulins (p = 0.0985), whereas isolates from Gauteng were more resistant to the tetracycyclines (p = <.0001). Poultry Campylobacter spp. were more prone to be resistant to enrofloxacin (p = 0.0021). Campylobacter jejuni, mainly isolated from poultry, was more liable to be resistant to the tetracyclines (chlrotetracycline p= 0.0307), whereas C. coli, predominatly isolated from pigs was more likely to be resistant to the macrolides (tylosin p= 0.063). Four of the bacteria isolated from the Western Cape were resistant to three or more antibiotic classes, namely; tetracyclines, macrolides, lincosamides, pleuromutulins and fluoroquinolones. No multi-resistant Campylobacter spp. were isolated from the flocks in Gauteng. With the exception of tiamulin, the bacterial populations could clearly be divided into resistant and susceptible populations. As consequence of the increased resistance to the antimicrobial classes used for human therapy and the geographical differences in antimicrobial susceptibility, it is recommended that an antimicrobial resistance monitoring system for the thermophilic Campylobacter spp. be initiated in the South Africa National Veterinary Surveillance and Monitoring Programme for Resistance to Antimicorbial Drugs (SANVAD) Copyright Veterinary Tropical Diseases unrestricted 2013-09-07T10:38:47Z 2010-08-10 2013-09-07T10:38:47Z 2010-04-16 2010-08-10 2010-08-10 Dissertation Jonker, A 2009, Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/27117 > E10/342/gm http://hdl.handle.net/2263/27117 http://upetd.up.ac.za/thesis/available/etd-08102010-165538/ © 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Poultry Broth microdilution Minimum inhibitory concentration Antimicrobial susceptibility Campylobacter coli Campylobacter jejuni Thermophilic Mic Pigs Gauteng Western Cape UCTD Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa |
| title | Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa |
| title_full | Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa |
| title_fullStr | Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa |
| title_full_unstemmed | Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa |
| title_short | Antimicrobial susceptibility in thermophilic Campylobacter species isolated from pigs and chickens in South Africa |
| title_sort | antimicrobial susceptibility in thermophilic campylobacter species isolated from pigs and chickens in south africa |
| topic | Poultry Broth microdilution Minimum inhibitory concentration Antimicrobial susceptibility Campylobacter coli Campylobacter jejuni Thermophilic Mic Pigs Gauteng Western Cape UCTD |
| url | http://hdl.handle.net/2263/27117 http://upetd.up.ac.za/thesis/available/etd-08102010-165538/ |