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Microbial community structure and dynamics within sulphate- removing bioreactors
Dissertation (MSc)--University of Pretoria, 2009.
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| Format: | Thesis |
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University of Pretoria
2013
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| _version_ | 1867613500430352384 |
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| access_status_str | Open Access |
| author2 | Venter, S.N. (Stephanus Nicolaas) |
| author_browse | Venter, S.N. (Stephanus Nicolaas) |
| author_facet | Venter, S.N. (Stephanus Nicolaas) |
| collection | Thesis |
| dc_rights_str_mv | © 2008, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2009. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/27197 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:08.286Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/27197 Microbial community structure and dynamics within sulphate- removing bioreactors Venter, S.N. (Stephanus Nicolaas) nicovanblerk@yahoo.com Van Blerk, Gerhardus Nicolas Water Pyrite rich soils Coal mining Oxygen UCTD Dissertation (MSc)--University of Pretoria, 2009. Mining activities, particularly coal mining, lead to the excavation of large volumes of pyrite rich soils. When exposed to air (oxygen) and water these pyrite complexes are oxidised to form highly acidic and corrosive wastewaters collectively termed acid mine drainage (AMD). Containing elevated levels of sulphates (SO42-) and toxic dissolved heavy metals, AMD seeping from mining sites, active or abandoned, poses a major environmental risk to aquatic bio-systems – not only in South Africa but globally. Chemical neutralization of AMD is expensive and often challenging. Biological sulphate reduction provides a promising and cheaper alternative to the treatment of sulphate rich wastewaters. Little, however, is known about the microbial communities involved in biological treatment systems and the effect of external factors thereon. Studying microorganisms in their natural environment is extremely difficult. The limitations of culture-based methods only provide a limited insight into the bacterial diversity of natural habitats and the microbial communities present. With the progressive advances in molecular biology, non culture-based tools such as DGGE, FISH and more recently t-RFLP allow easier and much more accurate studies of microbial communities within their natural as well as man-made environments. This study specifically investigated the use of t-RFLP to study microbial communities and dynamics within sulphate removing bioreactors. The set up and optimization of a t-RFLP system to specifically study microbial communities from sulphate removing bioreactors were investigated and the applicability of t-RFLP demonstrated. Copyright Microbiology and Plant Pathology unrestricted 2013-09-07T10:56:45Z 2009-09-16 2013-09-07T10:56:45Z 2009-04-15 2009-09-16 2009-08-12 Dissertation Van Blerk, GN 2008, Microbial community structure and dynamics within sulphate- removing bioreactors, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/27197 > E1367/gm http://hdl.handle.net/2263/27197 http://upetd.up.ac.za/thesis/available/etd-08122009-132505/ © 2008, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Water Pyrite rich soils Coal mining Oxygen UCTD Microbial community structure and dynamics within sulphate- removing bioreactors |
| title | Microbial community structure and dynamics within sulphate- removing bioreactors |
| title_full | Microbial community structure and dynamics within sulphate- removing bioreactors |
| title_fullStr | Microbial community structure and dynamics within sulphate- removing bioreactors |
| title_full_unstemmed | Microbial community structure and dynamics within sulphate- removing bioreactors |
| title_short | Microbial community structure and dynamics within sulphate- removing bioreactors |
| title_sort | microbial community structure and dynamics within sulphate removing bioreactors |
| topic | Water Pyrite rich soils Coal mining Oxygen UCTD |
| url | http://hdl.handle.net/2263/27197 http://upetd.up.ac.za/thesis/available/etd-08122009-132505/ |