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Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system
Dissertation (MSc (Microbiology))--University of Pretoria, 2007.
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University of Pretoria
2013
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| _version_ | 1867613673850142720 |
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| access_status_str | Open Access |
| author2 | Pietersen, Gerhard |
| author_browse | Pietersen, Gerhard |
| author_facet | Pietersen, Gerhard |
| collection | Thesis |
| dc_rights_str_mv | © 2006, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2007. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/27700 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:39:53.658Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/27700 Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system Pietersen, Gerhard jacolene@potatoseed.co.za/Jacolene.meyer@gmail.com Meyer, Jacolene Bee Banana Detection Antibody Development Badnavirus incidence System Transmission South africa Streak UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2007. Various research efforts have focused on Banana streak badnavirus (BSV), the causal agent of banana streak disease (BSD), since the discovery of endogenous sequences of the virus in the nuclear genome of several Musa (banana and plantain) species. In vitro propagation of Musa was identified as one of the main activation triggers of integrated BSV sequences to cause systemic (episomal) BSD. This was especially observed in B genome-containing tetraploid hybrids. Although, the South African banana industry is based on Cavendish varieties, some plantations with tetraploid hybrids have been established. In order to investigate the occurrence of episomally expressed BSV, a survey was done in the Kiepersol area of South Africa and episomal BSV was detected in six out of seven locations sampled. No episomal BSV was detected in the Cavendish cultivars sampled in close proximity to BSV infected cultivars. To determine the risk of vector-assisted spread of endogenous BSV, which has become episomally activated after tissue culture, transmission studies with local mealybug species (Planococcus citri (Risso), P. ficus (Signoret), Dysmicoccus brevipes (Cockerell) and Pseudococcus longispinus (Targioni-Tozzetti)) were conducted under controlled conditions. Virus-free FHIA-21 was multiplied in vitro and resulting progeny with, putatively episomally activated BSV, served as sources for mealybug-assisted transmissions to Cavendish. Activated, episomal BSV was transmitted by three mealybug species to Cavendish. Transmission with P. ficus was demonstrated for the first time. Limited antiserum stocks against BSV occur worldwide and detection of the virus remains crucial for the safe movement of Musa germplasm between continents. Antiserum is needed in order to detect the episomal form of the virus that causes BSD. Using conventional immunization methodology, antisera against a wide spectrum of BSV isolates were produced. Twenty diverse BSV isolates were characterized by IC-PCR and selected as sources for the production of the polyclonal antiserums in two animal species. An effective triple antibody sandwich (TAS) enzyme linked immunosorbent assay (ELISA) system; able to detect various serologically different species of BSV was developed. BSV was screened with a synthetically manufactured phage displayed antibody library; however, no satisfactory polyclonal or monoclonal antibodies were obtianed in using this approach. Microbiology and Plant Pathology unrestricted 2013-09-07T12:05:55Z 2007-02-09 2013-09-07T12:05:55Z 2006-05-02 2007-02-09 2007-02-09 Dissertation Meyer, J 2006, Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://hdl.handle.net/2263/27700 > http://hdl.handle.net/2263/27700 http://upetd.up.ac.za/thesis/available/etd-02092007-171659/ © 2006, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Banana Detection Antibody Development Badnavirus incidence System Transmission South africa Streak UCTD Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system |
| title | Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system |
| title_full | Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system |
| title_fullStr | Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system |
| title_full_unstemmed | Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system |
| title_short | Banana streak badnavirus (BSV) in South Africa : incidence, transmission and the development of an antibody based detection system |
| title_sort | banana streak badnavirus bsv in south africa incidence transmission and the development of an antibody based detection system |
| topic | Banana Detection Antibody Development Badnavirus incidence System Transmission South africa Streak UCTD |
| url | http://hdl.handle.net/2263/27700 http://upetd.up.ac.za/thesis/available/etd-02092007-171659/ |